Department of Radiotherapy and Radiation Oncology, University Medical Center Rostock, Suedring 75, 18059 Rostock, Germany.
Int J Mol Sci. 2022 Aug 1;23(15):8548. doi: 10.3390/ijms23158548.
In our study, our aim was to examine the cytotoxic and radio-sensitizing effect of the alkaloid piperine, a major pungent of black pepper, on two different human epithelial tumor cell lines in vitro. The growth of the human cell lines T98G (glioblastoma) and FaDu (hypopharyngeal carcinoma) was examined under the influence of piperine in different concentrations. In addition, after combined treatment with ionizing radiation, long-term survival was investigated with a colony formation assay. The proliferation was analyzed using the BrdU-assay, while the DNA repair capacity was examined via the γH2AX assay. Piperine reduced the growth of both cell lines in a concentration-dependent manner as well as a time-dependent one. After combined treatment with piperine and ionizing radiation, an inhibition of clonogenic survival could be proven. A reduced proliferation capacity and an additive effect on DNA damage 24 h after irradiation are possible causal mechanisms, which were also demonstrated for both cell lines. Based on the results presented in this study, piperine was shown to have cytotoxic antitumor activity and a radio-sensitizing effect in micromolar concentrations in the human tumor cells that were tested. Based on these results piperine represents a potential therapeutic option in radio-oncological treatment.
在我们的研究中，我们的目的是研究生物碱胡椒碱（黑胡椒的主要刺激性成分）对两种不同的人上皮肿瘤细胞系在体外的细胞毒性和放射增敏作用。在不同浓度的胡椒碱的影响下，研究了人细胞系 T98G（胶质母细胞瘤）和 FaDu（下咽癌）的生长情况。此外，通过集落形成测定法研究了联合电离辐射后的长期生存情况。通过 BrdU 测定法分析了增殖情况，通过 γH2AX 测定法检查了 DNA 修复能力。胡椒碱以浓度和时间依赖性方式降低了两种细胞系的生长。胡椒碱联合电离辐射处理后，可抑制集落形成存活。减少增殖能力和对照射后 24 小时 DNA 损伤的附加作用可能是两种细胞系都存在的潜在因果机制。基于本研究中提出的结果，胡椒碱在测试的人类肿瘤细胞中以微摩尔浓度显示出细胞毒性抗肿瘤活性和放射增敏作用。基于这些结果，胡椒碱代表了放射肿瘤治疗中潜在的治疗选择。
