Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
Institute of Plasma Technology, Korea Institute of Fusion Energy, 37, Dongjangsan-ro, Gunsan-si 54004, Korea.
Int J Mol Sci. 2022 Aug 4;23(15):8665. doi: 10.3390/ijms23158665.
In plants, vegetative and reproductive development are associated with agronomically important traits that contribute to grain yield and biomass. Zinc finger homeodomain (ZF-HD) transcription factors (TFs) constitute a relatively small gene family that has been studied in several model plants, including L. and L. The ZF-HD family members play important roles in plant growth and development, but their contribution to the regulation of plant architecture remains largely unknown due to their functional redundancy. To understand the gene regulatory network controlled by ZF-HD TFs, we analyzed multiple loss-of-function mutants of ZF-HD TFs in that exhibited morphological abnormalities in branching and flowering architecture. We found that ZF-HD TFs, especially HB34, negatively regulate the expression of miR157 and positively regulate SQUAMOSA PROMOTER BINDING-LIKE 10 (), a target of miR157. Genome-wide chromatin immunoprecipitation sequencing (ChIP-Seq) analysis revealed that and are direct targets of HB34, creating a feed-forward loop that constitutes a robust miRNA regulatory module. Network motif analysis contains overrepresented coherent type IV feedforward motifs in the amiR zf-HD and mutant background. This finding indicates that miRNA-mediated ZF-HD feedforward modules modify branching and inflorescence architecture in . Taken together, these findings reveal a guiding role of ZF-HD TFs in the regulatory network module and demonstrate its role in plant architecture in .
在植物中，营养生长和生殖生长与具有重要农艺性状的特征相关，这些特征有助于提高谷物产量和生物量。锌指结构域 homeodomain (ZF-HD) 转录因子 (TF) 构成一个相对较小的基因家族，已在包括拟南芥和玉米在内的几种模式植物中进行了研究。ZF-HD 家族成员在植物生长和发育中发挥重要作用，但由于其功能冗余，它们对植物结构调控的贡献在很大程度上仍不清楚。为了了解 ZF-HD TF 调控的基因调控网络，我们分析了 中多个 ZF-HD TF 的功能丧失突变体，这些突变体在分枝和开花结构上表现出形态异常。我们发现 ZF-HD TFs，特别是 HB34，负调控 miR157 的表达，并正调控 SQUAMOSA PROMOTER BINDING-LIKE 10 ()，miR157 的靶标。全基因组染色质免疫沉淀测序 (ChIP-Seq) 分析表明 和 是 HB34 的直接靶标，形成一个正反馈环，构成一个稳健的 miRNA 调控模块。网络 motif 分析表明，在 amiR zf-HD 和 突变体背景下，存在过度表达的一致型 IV 正反馈 motif。这一发现表明，miRNA 介导的 ZF-HD 正反馈模块修饰了 的分枝和花序结构。总之，这些发现揭示了 ZF-HD TFs 在调控网络模块中的指导作用，并证明了其在 的植物结构中的作用。
