Department of Human Enhancement and Hand Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Yanagido, Gifu 501-1193, Japan.
Int J Mol Sci. 2022 Aug 7;23(15):8773. doi: 10.3390/ijms23158773.
Neural cell transplantation targeting peripheral nerves is a potential treatment regime for denervated muscle atrophy. This study aimed to develop a new therapeutic technique for intractable muscle atrophy by the xenotransplantation of neural stem cells derived from pig fetuses into peripheral nerves. In this study, we created a denervation model using neurotomy in nude rats and transplanted pig-fetus-derived neural stem cells into the cut nerve stump. Three months after transplantation, the survival of neural cells, the number and area of regenerated axons, and the degree of functional recovery by electrical stimulation of peripheral nerves were compared among the gestational ages (E 22, E 27, E 45) of the pigs. Transplanted neural cells were engrafted at all ages. Functional recovery by electric stimulation was observed at age E 22 and E 27. This study shows that the xenotransplantation of fetal porcine neural stem cells can restore denervated muscle function. When combined with medical engineering, this technology can help in developing a new therapy for paralysis.
神经细胞移植靶向外周神经是治疗去神经肌肉萎缩的一种有潜力的治疗方案。本研究旨在通过将源自猪胎儿的神经干细胞移植到外周神经中来开发一种治疗难治性肌肉萎缩的新治疗技术。在这项研究中，我们使用裸鼠的神经切断术创建了一个去神经模型，并将猪胎源性神经干细胞移植到切断的神经残端。移植后 3 个月，比较了来自不同胎龄（E22、E27、E45）猪的神经细胞的存活、再生轴突的数量和面积，以及对外周神经电刺激的功能恢复程度。在所有胎龄中都有移植的神经细胞。在 E22 和 E27 年龄观察到电刺激的功能恢复。本研究表明，胎猪神经干细胞的异种移植可以恢复去神经肌肉的功能。当与医学工程结合时，这项技术可以帮助开发一种新的瘫痪治疗方法。
