Mergenhagen D, Mergenhagen E
Eur J Cell Biol. 1987 Apr;43(2):203-7.
The overt circadian rhythm in a wildtype (wt+) and a short period (s-) strain of Chlamydomonas reinhardii has been studied in space using the photoaccumulation behavior as the recorded parameter. The period of the wt+ was 29.6 h, of the s- 21.4 h and did not deviate significantly from ground controls performed exactly at the same time. The phase was delayed in space by 4.2 h in the wt+, but was not altered in the s-. In both strains the amplitudes were significantly higher in space than in the ground controls. During the recording period of 6.5 days the cell density increased in both strains. The survival rate, i.e. the ability to form colonies on agar petri dishes, was higher in space than on ground. The period was in both strains by 1.1 h longer in Florida (Kennedy Space Center) in both the flight and the control samples than in Europe. The significance of these results is discussed with respect to the endogenous nature of the biological clock and the role of the microgravity environment.
利用光积累行为作为记录参数，在太空中研究了莱茵衣藻野生型（wt+）和短周期（s-）菌株的明显昼夜节律。wt+的周期为29.6小时，s-的周期为21.4小时，与同时进行的地面对照相比，差异不显著。wt+在太空中的相位延迟了4.2小时，但s-的相位没有改变。在两种菌株中，太空中的振幅均显著高于地面对照。在6.5天的记录期内，两种菌株的细胞密度均增加。在太空中的存活率，即在琼脂培养皿上形成菌落的能力，高于地面。在佛罗里达州（肯尼迪航天中心），飞行样本和对照样本中两种菌株的周期都比在欧洲时长1.1小时。结合生物钟的内源性本质和微重力环境的作用对这些结果的意义进行了讨论。
