• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于纳米材料危害评估的先进呼吸模型——单培养、共培养和三培养的性能

Advanced Respiratory Models for Hazard Assessment of Nanomaterials-Performance of Mono-, Co- and Tricultures.

作者信息

Camassa Laura Maria Azzurra, Elje Elisabeth, Mariussen Espen, Longhin Eleonora Marta, Dusinska Maria, Zienolddiny-Narui Shan, Rundén-Pran Elise

机构信息

National Institute of Occupational Health in Norway, 0033 Oslo, Norway.

NILU-Norwegian Institute for Air Research, 2027 Kjeller, Norway.

出版信息

Nanomaterials (Basel). 2022 Jul 29;12(15):2609. doi: 10.3390/nano12152609.

DOI:10.3390/nano12152609
PMID:35957046
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9370172/
Abstract

Advanced in vitro models are needed to support next-generation risk assessment (NGRA), moving from hazard assessment based mainly on animal studies to the application of new alternative methods (NAMs). Advanced models must be tested for hazard assessment of nanomaterials (NMs). The aim of this study was to perform an interlaboratory trial across two laboratories to test the robustness of and optimize a 3D lung model of human epithelial A549 cells cultivated at the air-liquid interface (ALI). Potential change in sensitivity in hazard identification when adding complexity, going from monocultures to co- and tricultures, was tested by including human endothelial cells EA.hy926 and differentiated monocytes dTHP-1. All models were exposed to NM-300K in an aerosol exposure system (VITROCELL cloud-chamber). Cyto- and genotoxicity were measured by AlamarBlue and comet assay. Cellular uptake was investigated with transmission electron microscopy. The models were characterized by confocal microscopy and barrier function tested. We demonstrated that this advanced lung model is applicable for hazard assessment of NMs. The results point to a change in sensitivity of the model by adding complexity and to the importance of detailed protocols for robustness and reproducibility of advanced in vitro models.

摘要

需要先进的体外模型来支持下一代风险评估（NGRA），即从主要基于动物研究的危害评估转向应用新的替代方法（NAMs）。先进的模型必须针对纳米材料（NMs）的危害评估进行测试。本研究的目的是在两个实验室之间开展一项实验室间试验，以测试在气液界面（ALI）培养的人上皮A549细胞三维肺模型的稳健性并对其进行优化。通过纳入人内皮细胞EA.hy926和分化的单核细胞dTHP-1，测试了从单一培养到共培养和三培养增加复杂性时危害识别敏感性的潜在变化。所有模型在气溶胶暴露系统（VITROCELL云室）中暴露于NM-300K。通过AlamarBlue和彗星试验测量细胞毒性和遗传毒性。用透射电子显微镜研究细胞摄取。通过共聚焦显微镜对模型进行表征并测试屏障功能。我们证明了这种先进的肺模型适用于纳米材料的危害评估。结果表明，增加复杂性会改变模型的敏感性，也表明详细方案对于先进体外模型的稳健性和可重复性的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/c070f0acccd3/nanomaterials-12-02609-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/e0db654e5f09/nanomaterials-12-02609-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/111fc4e5ef49/nanomaterials-12-02609-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/de27e0d96340/nanomaterials-12-02609-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/fc852435c676/nanomaterials-12-02609-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/f459545154e9/nanomaterials-12-02609-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/84597cc9ca21/nanomaterials-12-02609-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/bb3c76758d37/nanomaterials-12-02609-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/c070f0acccd3/nanomaterials-12-02609-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/e0db654e5f09/nanomaterials-12-02609-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/111fc4e5ef49/nanomaterials-12-02609-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/de27e0d96340/nanomaterials-12-02609-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/fc852435c676/nanomaterials-12-02609-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/f459545154e9/nanomaterials-12-02609-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/84597cc9ca21/nanomaterials-12-02609-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/bb3c76758d37/nanomaterials-12-02609-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fea/9370172/c070f0acccd3/nanomaterials-12-02609-g008.jpg

相似文献

1
Advanced Respiratory Models for Hazard Assessment of Nanomaterials-Performance of Mono-, Co- and Tricultures.用于纳米材料危害评估的先进呼吸模型——单培养、共培养和三培养的性能
Nanomaterials (Basel). 2022 Jul 29;12(15):2609. doi: 10.3390/nano12152609.
2
Different Sensitivity of Advanced Bronchial and Alveolar Mono- and Coculture Models for Hazard Assessment of Nanomaterials.先进的支气管和肺泡单培养及共培养模型对纳米材料危害评估的不同敏感性
Nanomaterials (Basel). 2023 Jan 19;13(3):407. doi: 10.3390/nano13030407.
3
Transferability and reproducibility of exposed air-liquid interface co-culture lung models.暴露气液界面共培养肺模型的可转移性和可重复性。
NanoImpact. 2023 Jul;31:100466. doi: 10.1016/j.impact.2023.100466. Epub 2023 May 18.
4
An inter-laboratory effort to harmonize the cell-delivered in vitro dose of aerosolized materials.一项协调细胞递送的雾化材料体外剂量的实验室间工作。
NanoImpact. 2022 Oct;28:100439. doi: 10.1016/j.impact.2022.100439. Epub 2022 Nov 17.
5
Gene Expression Profiling of Mono- and Co-Culture Models of the Respiratory Tract Exposed to Crystalline Quartz under Submerged and Air-Liquid Interface Conditions.呼吸道暴露于浸液和气-液界面条件下的结晶石英的单培养和共培养模型的基因表达谱分析。
Int J Mol Sci. 2022 Jul 14;23(14):7773. doi: 10.3390/ijms23147773.
6
Quartz crystal microbalances (QCM) are suitable for real-time dosimetry in nanotoxicological studies using VITROCELL®Cloud cell exposure systems.石英晶体微天平（QCM）适用于使用 VITROCELL®Cloud 细胞暴露系统进行纳米毒理学研究的实时剂量测定。
Part Fibre Toxicol. 2020 Sep 16;17(1):44. doi: 10.1186/s12989-020-00376-w.
7
An integrated new approach methodology for inhalation risk assessment of safe and sustainable by design nanomaterials.一种用于安全和可持续设计纳米材料吸入风险评估的综合新方法。
Environ Int. 2024 Jan;183:108420. doi: 10.1016/j.envint.2024.108420. Epub 2024 Jan 3.
8
Air-liquid interface exposure to aerosols of poorly soluble nanomaterials induces different biological activation levels compared to exposure to suspensions.与暴露于悬浮液相比，气液界面暴露于难溶性纳米材料的气溶胶会诱导不同水平的生物活化。
Part Fibre Toxicol. 2016 Nov 3;13(1):58. doi: 10.1186/s12989-016-0171-3.
9
An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols.体外肺系统评估碳纳米管气溶胶的促炎危害。
Int J Mol Sci. 2020 Jul 27;21(15):5335. doi: 10.3390/ijms21155335.
10
Genetic toxicity assessment of engineered nanoparticles using a 3D in vitro skin model (EpiDerm™).使用3D体外皮肤模型（EpiDerm™）对工程纳米颗粒进行遗传毒性评估。
Part Fibre Toxicol. 2016 Sep 9;13(1):50. doi: 10.1186/s12989-016-0161-5.

引用本文的文献

1
Hazard assessment of nanomaterials: how to meet the requirements for (next generation) risk assessment.纳米材料的危害评估：如何满足（下一代）风险评估的要求。
Part Fibre Toxicol. 2024 Dec 27;21(1):54. doi: 10.1186/s12989-024-00615-4.
2
Toward Standardization of a Lung New Approach Model for Toxicity Testing of Nanomaterials.迈向纳米材料毒性测试肺新方法模型的标准化
Nanomaterials (Basel). 2024 Nov 24;14(23):1888. doi: 10.3390/nano14231888.
3
Impact of Wildfires on Cardiovascular Health.野火对心血管健康的影响。

本文引用的文献

1
Lung Models to Evaluate Silver Nanoparticles' Toxicity and Their Impact on Human Health.用于评估银纳米颗粒毒性及其对人类健康影响的肺部模型
Nanomaterials (Basel). 2022 Jul 5;12(13):2316. doi: 10.3390/nano12132316.
2
Inter-laboratory variability of A549 epithelial cells grown under submerged and air-liquid interface conditions.A549 上皮细胞在浸没和气液界面条件下培养的实验室间变异性。
Toxicol In Vitro. 2021 Sep;75:105178. doi: 10.1016/j.tiv.2021.105178. Epub 2021 Apr 24.
3
Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure.
Circ Res. 2024 Apr 26;134(9):1061-1082. doi: 10.1161/CIRCRESAHA.124.323614. Epub 2024 Apr 25.
4
New approach methodologies to enhance human health risk assessment of immunotoxic properties of chemicals - a PARC (Partnership for the Assessment of Risk from Chemicals) project.增强化学品免疫毒性特性人体健康风险评估的新方法学——化学品风险评估合作组织（PARC）项目
Front Toxicol. 2024 Apr 9;6:1339104. doi: 10.3389/ftox.2024.1339104. eCollection 2024.
5
Different Sensitivity of Advanced Bronchial and Alveolar Mono- and Coculture Models for Hazard Assessment of Nanomaterials.先进的支气管和肺泡单培养及共培养模型对纳米材料危害评估的不同敏感性
Nanomaterials (Basel). 2023 Jan 19;13(3):407. doi: 10.3390/nano13030407.
在细胞培养模型中对吸入性纳米颗粒进行长期暴露影响的筛选。
Nanomaterials (Basel). 2021 Feb 28;11(3):606. doi: 10.3390/nano11030606.
4
Determination of Genotoxicity Attributed to Diesel Exhaust Particles in Normal Human Embryonic Lung Cell (WI-38) Line.测定正常人体胚胎肺细胞（WI-38 细胞）系中归因于柴油废气颗粒的遗传毒性。
Biomolecules. 2021 Feb 16;11(2):291. doi: 10.3390/biom11020291.
5
Optimization of an air-liquid interface cell co-culture model to estimate the hazard of aerosol exposures.优化气液界面细胞共培养模型以评估气溶胶暴露的危害。
J Aerosol Sci. 2021 Mar;153:105703. doi: 10.1016/j.jaerosci.2020.105703.
6
Research progress on the carcinogenicity of metal nanomaterials.金属纳米材料致癌性的研究进展
J Appl Toxicol. 2021 Sep;41(9):1334-1344. doi: 10.1002/jat.4145. Epub 2021 Feb 1.
7
Nanomaterials in the environment, human exposure pathway, and health effects: A review.环境中的纳米材料、人体暴露途径和健康影响：综述。
Sci Total Environ. 2021 Mar 10;759:143470. doi: 10.1016/j.scitotenv.2020.143470. Epub 2020 Nov 17.
8
Quartz crystal microbalances (QCM) are suitable for real-time dosimetry in nanotoxicological studies using VITROCELL®Cloud cell exposure systems.石英晶体微天平（QCM）适用于使用 VITROCELL®Cloud 细胞暴露系统进行纳米毒理学研究的实时剂量测定。
Part Fibre Toxicol. 2020 Sep 16;17(1):44. doi: 10.1186/s12989-020-00376-w.
9
An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols.体外肺系统评估碳纳米管气溶胶的促炎危害。
Int J Mol Sci. 2020 Jul 27;21(15):5335. doi: 10.3390/ijms21155335.
10
Thermodynamic Parameters at Bio-Nano Interface and Nanomaterial Toxicity: A Case Study on BSA Interaction with ZnO, SiO, and TiO.生物-纳米界面的热力学参数与纳米材料毒性：以 BSA 与 ZnO、SiO 和 TiO 的相互作用为例。
Chem Res Toxicol. 2020 Aug 17;33(8):2054-2071. doi: 10.1021/acs.chemrestox.9b00468. Epub 2020 Jul 15.