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一种冷冻同步有丝分裂细胞和G1期细胞的方法。

A method for freezing synchronous mitotic and G1 cells.

作者信息

Borrelli M J, Mackey M A, Dewey W C

出版信息

Exp Cell Res. 1987 Jun;170(2):363-8. doi: 10.1016/0014-4827(87)90313-2.

DOI:10.1016/0014-4827(87)90313-2
PMID:3595736
Abstract

A modification of the protocol developed by Kawamoto, J C & Barrett, J N, Brain res (1986), in press for freezing primary neuron cultures in a solution containing low sodium and high lactate and potassium concentrations was used to freeze synchronous mitotic and G1 CHO cells. After thawing, the cells behaved as if they had never been frozen with respect to cell growth, cell division, plating efficiency, and hyperthermic sensitivity.

摘要

采用了由川本(Kawamoto, J C)和巴雷特(Barrett, J N)开发的方案的一种改进方法(《脑研究》(Brain res),1986年,即将发表),该方案用于在含有低钠、高乳酸和高钾浓度的溶液中冷冻原代神经元培养物,以此来冷冻同步有丝分裂期和G1期的中国仓鼠卵巢(CHO)细胞。解冻后,这些细胞在细胞生长、细胞分裂、接种效率和热敏感性方面的表现就如同从未被冷冻过一样。

相似文献

1
A method for freezing synchronous mitotic and G1 cells.一种冷冻同步有丝分裂细胞和G1期细胞的方法。
Exp Cell Res. 1987 Jun;170(2):363-8. doi: 10.1016/0014-4827(87)90313-2.
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Protocol for freezing thermotolerant cells and maintaining thermotolerance following thawing.耐热细胞冷冻及解冻后维持耐热性的方案。
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引用本文的文献

1
Position-dependent transcriptional regulation of the murine dihydrofolate reductase promoter by the E2F transactivation domain.E2F反式激活结构域对小鼠二氢叶酸还原酶启动子的位置依赖性转录调控
Mol Cell Biol. 1997 Apr;17(4):1966-76. doi: 10.1128/MCB.17.4.1966.
2
Sequences downstream of the transcription initiation site modulate the activity of the murine dihydrofolate reductase promoter.转录起始位点下游的序列调节小鼠二氢叶酸还原酶启动子的活性。
Mol Cell Biol. 1990 Apr;10(4):1390-8. doi: 10.1128/mcb.10.4.1390-1398.1990.
3
Transcription initiation from the dihydrofolate reductase promoter is positioned by HIP1 binding at the initiation site.
二氢叶酸还原酶启动子的转录起始是通过HIP1在起始位点的结合来定位的。
Mol Cell Biol. 1990 Feb;10(2):653-61. doi: 10.1128/mcb.10.2.653-661.1990.
4
The HIP1 initiator element plays a role in determining the in vitro requirement of the dihydrofolate reductase gene promoter for the C-terminal domain of RNA polymerase II.HIP1起始元件在确定二氢叶酸还原酶基因启动子对RNA聚合酶II C末端结构域的体外需求方面发挥作用。
Mol Cell Biol. 1992 May;12(5):2250-9. doi: 10.1128/mcb.12.5.2250-2259.1992.
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The HIP1 binding site is required for growth regulation of the dihydrofolate reductase gene promoter.二氢叶酸还原酶基因启动子的生长调节需要HIP1结合位点。
Mol Cell Biol. 1992 Mar;12(3):1054-63. doi: 10.1128/mcb.12.3.1054-1063.1992.