Straneva J E, Yang H H, Hui S L, Bruno E, Hoffman R
Exp Hematol. 1987 Jul;15(6):657-63.
Suspensions of enriched human megakaryocytes (MK) devoid of MK progenitors (CFU-MK) undergo complete cytoplasmic maturation in vitro. MK were cultured in the presence of normal human AB serum (NABS) to mimic "normal" development. The rate of maturation was not statistically altered by higher concentrations (10%-20%-30%) of NABS, or by the addition of bovine serum albumin (1.5%-3.0%), but was accelerated in the presence of aplastic anemia serum (AAS). Sera from eight different patients with severe aplastic anemia were effective in accelerating terminal differentiation. MK-CSF, a glycoprotein isolated from AAS, specifically augments MK colony formation by two- to sixfold. Similar doses of MK-CSF were ineffective in altering terminal cytoplasmic maturation. Anti-MK-CSF, a polyclonal antibody prepared against purified MK-CSF, neutralizes the ability of both purified MK-CSF and AAS to promote MK colony formation. However, AAS adsorbed with anti-MK-CSF still retained its ability to accelerate terminal differentiation. Apparently, AAS contains at least two separate humoral factors, which can regulate in vitro human megakaryocytopoiesis: MK-CSF, which stimulates proliferation of the progenitors (CFU-MK), and a maturation factor, which accelerates cytoplasmic maturation of morphologically recognizable megakaryocytes.
不含巨核细胞祖细胞（CFU-MK）的富集人巨核细胞（MK）悬液在体外可完成细胞质成熟。将MK在正常人AB血清（NABS）存在的情况下进行培养，以模拟“正常”发育过程。较高浓度（10%-20%-30%）的NABS或添加牛血清白蛋白（1.5%-3.0%）并不会使成熟速率发生统计学改变，但再生障碍性贫血血清（AAS）会加速其成熟。来自8名不同严重再生障碍性贫血患者的血清均能有效加速终末分化。从AAS中分离出的一种糖蛋白——巨核细胞集落刺激因子（MK-CSF）可使MK集落形成特异性增加2至6倍。相似剂量的MK-CSF在改变终末细胞质成熟方面无效。抗MK-CSF是一种针对纯化的MK-CSF制备的多克隆抗体，它能中和纯化的MK-CSF和AAS促进MK集落形成的能力。然而，用抗MK-CSF吸附过的AAS仍保留其加速终末分化的能力。显然，AAS至少包含两种独立的体液因子，它们可在体外调节人巨核细胞生成：刺激祖细胞（CFU-MK）增殖的MK-CSF，以及加速形态上可识别的巨核细胞细胞质成熟的成熟因子。
