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巨型蟋蟀(直翅目:蟋蟀科)的完整线粒体基因组及其注释

The complete mitochondrial genome of giant cricket, (Orthoptera: Gryllidae) and its curation.

作者信息

Homchan Somjit, Gupta Yash Munnalal

机构信息

Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand.

出版信息

Mitochondrial DNA B Resour. 2022 Aug 5;7(8):1427-1431. doi: 10.1080/23802359.2022.2107441. eCollection 2022.

Abstract

, commonly known as giant cricket one of the important edible cricket species. However, the genetic information of these species is still limited. Therefore, we have assembled and annotated the first mitochondrial genome of . The mitogenome is 15710 bp long and has GC content of 27.19%. The nucleotide composition is similar with other insect mitogenomes (A 40.6%; T 32.2%; C 17.3%; G 9.9%). The gene organization in the mitogenome of is identical to the mitogenome of other cricket species. The complete mitogenome of consisted 37 genes including 13 protein coding genes, 22 tRNA genes, and two rRNA genes. The newly assembled mitogenome will help molecular biology research on edible crickets. Since mitogenome genes are traditionally used for DNA barcoding and phylogenetic analysis, comparative analysis of mitogenome with other related cricket species will also aid researchers in developing universal primers for species identification toward food security. Apart from the main goal of providing full mitogenome of paper also provides conceptual workflow based on assembly and its correction for final mitogenome construction.

摘要

,通常被称为巨型蟋蟀,是重要的可食用蟋蟀物种之一。然而,这些物种的遗传信息仍然有限。因此,我们组装并注释了首个 的线粒体基因组。该线粒体基因组长度为15710 bp,GC含量为27.19%。核苷酸组成与其他昆虫线粒体基因组相似(A 40.6%;T 32.2%;C 17.3%;G 9.9%)。 的线粒体基因组中的基因组织与其他蟋蟀物种的线粒体基因组相同。 的完整线粒体基因组由37个基因组成,包括13个蛋白质编码基因、22个tRNA基因和两个rRNA基因。新组装的线粒体基因组将有助于可食用蟋蟀的分子生物学研究。由于线粒体基因组基因传统上用于DNA条形码和系统发育分析,将 的线粒体基因组与其他相关蟋蟀物种进行比较分析也将有助于研究人员开发用于物种鉴定的通用引物,以保障食品安全。除了提供 的完整线粒体基因组这一主要目标外,本文还提供了基于 组装及其校正以构建最终线粒体基因组的概念性工作流程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de90/9359167/d2f1a30b61ac/TMDN_A_2107441_F0001_C.jpg

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