Dong Jiajia, Vicente Natallia, Chintauan-Marquier Ioana C, Ramadi Cahyo, Dettai Agnès, Robillard Tony
College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, P.R. China. Muséum national d'Histoire naturelle, Institut de Systématique, Evolution et Biodiversité ISYEB, UMR 7205 CNRS MNHN UPMC EPHE CP 50 (Entomologie), Sorbonne Universités, 75231 Paris Cedex 05, France..
Zootaxa. 2017 May 15;4268(1):101-116. doi: 10.11646/zootaxa.4268.1.6.
In the present study, we report the high-coverage complete mitochondrial genome (mitogenome) of the cricket Cardiodactylus muiri Otte, 2007. The mitogenome was sequenced using a long-PCR approach on an Ion Torrent Personal Genome Machine (PGM) for next generation sequencing technology. The total length of the amplified mitogenome is 16,328 bp, representing 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one noncoding region (D-loop region). The new sets of long-PCR primers reported here are invaluable resources for future comparative evolutionary genomic studies in Orthopteran insects. The new mitogenome sequence is compared with published cricket mitogenomes. In the taxonomic part, we present new records for the species and describe life-history traits, habitat and male calling song of the species; based on observation of new material, the species Cardiodactylus buru Gorochov & Robillard, 2014 is synonymized under C. muiri.
在本研究中，我们报道了2007年穆氏心形指突蟋（Cardiodactylus muiri Otte）的高覆盖度完整线粒体基因组（线粒体基因组）。该线粒体基因组采用长PCR方法，在Ion Torrent个人基因组测序仪（PGM）上进行下一代测序技术测序。扩增得到的线粒体基因组全长16328 bp，包含13个蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因和1个非编码区（D环区）。本文报道的新的长PCR引物组是直翅目昆虫未来比较进化基因组学研究的宝贵资源。将新的线粒体基因组序列与已发表的蟋蟀线粒体基因组进行了比较。在分类部分，我们给出了该物种的新记录，并描述了该物种的生活史特征、栖息地和雄性鸣声；基于对新材料的观察，2014年的布鲁心形指突蟋（Cardiodactylus buru Gorochov & Robillard）被认定为穆氏心形指突蟋的同物异名。
