氢化可的松对体外人红细胞生成的调节作用。

Modulation of human erythropoiesis by hydrocortisone in vitro.

作者信息

King D J, Koekebakker M, Barr R D

出版信息

Eur J Haematol. 1987 Feb;38(2):137-40. doi: 10.1111/j.1600-0609.1987.tb01151.x.

DOI:10.1111/j.1600-0609.1987.tb01151.x

Abstract

The effect of hydrocortisone on the formation of erythroid colonies was studied in vitro in cultures of normal human bone marrow in an agar system. A range of concentrations of hydrocortisone (10(-10) to 10(-3) mol/l) showed significant effects on erythroid burst formation, in terms of the number of colonies, on d 10 and 14 of culture. At subphysiological concentrations (10(-10) to 10(-8) mol/l), no effect was seen, but at both physiological (10(-7) mol/l) and pharmacological (10(-6) and 10(-5) mol/l) concentrations stimulation of erythroid burst formation was noted. At 10(-4) mol/l hydrocortisone inhibited erythroid colony formation and 10(-3) mol/l was uniformly lethal. In the concentration range of 10(-7) to 10(-5) mol/l hydrocortisone also appeared to increase erythroid colony size. Thus hydrocortisone (10(-7) to 10(-5) mol/l) stimulates erythroid colony growth and it is suggested that the hormone may play a role in the physiological regulation of human erythropoiesis.

摘要

在琼脂体系中对正常人骨髓培养物进行体外研究，以探讨氢化可的松对红系集落形成的影响。一系列浓度的氢化可的松（10⁻¹⁰至10⁻³mol/L）对培养第10天和第14天的红系爆式集落形成（以集落数量计）显示出显著影响。在亚生理浓度（10⁻¹⁰至10⁻⁸mol/L）下未观察到影响，但在生理浓度（10⁻⁷mol/L）和药理浓度（10⁻⁶和10⁻⁵mol/L）下均注意到对红系爆式集落形成有刺激作用。在10⁻⁴mol/L时氢化可的松抑制红系集落形成，而10⁻³mol/L具有一致的致死性。在10⁻⁷至10⁻⁵mol/L浓度范围内，氢化可的松似乎还能增加红系集落大小。因此，氢化可的松（10⁻⁷至10⁻⁵mol/L）刺激红系集落生长，提示该激素可能在人类红细胞生成的生理调节中起作用。