Zhao Irene Y, Jung Jiin, Moyne Anne-Laure, Schaffner Donald W, Harris Linda J
Department of Food Science and Technology University of California, Davis Davis California USA.
Western Center for Food Safety University of California, Davis Davis California USA.
JSFA Rep. 2021 Dec;1(1):17-25. doi: 10.1002/jsf2.21. Epub 2021 Nov 4.
Both reusable and single-use gloves can be employed during hand harvesting of lettuce and leafy greens. The impact of glove type on survival and transfer of was evaluated using agar or lettuce in a laboratory setting and during simulated lettuce harvesting in the field.
Textured and smooth reusable latex and smooth disposable latex gloves inoculated with were sequentially touched to 10 or 20 agar plates or 20 lettuce leaves ( = 6; laboratory) or used to sequentially harvest 20 heads of lettuce ( = 6; field). was recovered by enrichment from significantly fewer leaves (46%; 55 of 120) or heads (26%; 31 of 120) of lettuce when inoculated reusable textured gloves were used compared with disposable gloves (leaves: 98%; 118 of 120, or heads: 74%; 89 of 120). In contrast, when a single head of lettuce was the point source for glove contamination, there was no significant difference in the number of -positive lettuce heads harvested with reusable textured (71%; 85 of 120) or disposable gloves (75%; 90 of 120). In either field-contamination scenario, at the 20th head of lettuce harvested with a single glove (final sample point), was recovered from one to five of six lettuce heads across experimental trials.
Contamination of a glove from a single point source can lead to subsequent contamination of multiple heads of lettuce during hand harvesting, showing the importance of policies to manage hand hygiene and glove use for harvest crews.
在人工采收生菜和叶类蔬菜时，可使用可重复使用的手套和一次性手套。在实验室环境以及田间模拟生菜采收过程中，使用琼脂或生菜评估了手套类型对[具体微生物名称未给出]存活和转移的影响。
接种了[具体微生物名称未给出]的有纹理和光滑的可重复使用乳胶手套以及光滑的一次性乳胶手套，依次接触10个或20个琼脂平板或20片生菜叶（n = 6；实验室），或用于依次采收20颗生菜（n = 6；田间）。与一次性手套（叶片：98%；120片中有118片，或菜头：74%；120颗中有89颗）相比，使用接种了[具体微生物名称未给出]的可重复使用有纹理手套时，从生菜叶片（46%；120片中的55片）或菜头（26%；120颗中的31颗）中通过富集回收的[具体微生物名称未给出]显著减少。相比之下，当单个生菜头是手套污染的源头时，使用可重复使用有纹理手套（71%；120颗中的85颗）或一次性手套（75%；120颗中的90颗）采收的被[具体微生物名称未给出]污染的生菜头数量没有显著差异。在任何一种田间污染情况下，在使用单个手套采收的第20颗生菜头（最终采样点）时，在各实验中，6颗生菜头中有1至5颗检测到[具体微生物名称未给出]。
手套从单个源头受到污染会导致人工采收过程中多个生菜头随后受到污染，这表明管理采收人员手部卫生和手套使用政策的重要性。
