Yin Shenggang, You Ting, Tang Jiayong, Wang Longqiong, Jia Gang, Liu Guangmang, Tian Gang, Chen Xiaoling, Cai Jingyi, Kang Bo, Zhao Hua
Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Front Vet Sci. 2022 Jul 26;9:942253. doi: 10.3389/fvets.2022.942253. eCollection 2022.
Weaning often induces oxidative stress and inflammatory response in piglets. This study investigated the effects of dietary licorice flavonoids powder (LFP) supplementation on antioxidant capacity and immunity in weaned piglets. Notably, 96 Landrace × Yorkshire × Duroc (DLY) weaned piglets were randomly allocated to four treatments with 6 replicates (4 animals per replicate) and fed with diet supplementation with 0, 50, 150, and 250 mg/kg LFP, respectively. The trial lasted for 5 weeks. The results showed that dietary LFP supplementation effectively increased the liver index ( < 0.05). In addition, dietary LFP supplementation reduced serum aspartate aminotransferase activity ( < 0.01). Piglets fed with 50 mg/kg LFP decreased total cholesterol and HDL-C content in serum ( < 0.05) and increased serum alkaline phosphatase activity ( < 0.01). Similarly, supplementation with 150 mg/kg LFP elevated the activity of total antioxidant capability (T-AOC) in serum ( < 0.01) and dietary with 150 and 250 mg/kg LFP increased T-AOC activity in spleen ( < 0.01). Moreover, dietary with 150 mg/kg LFP addition enhanced ( < 0.05) the serum IgG content of piglets. Additionally, compared with the control group, dietary 250 mg/kg LFP supplementation upregulated ( < 0.05) the mRNA abundance of (β and in the spleen. Meanwhile, dietary 150 and 250 mg/kg LFP supplementation downregulated ( < 0.05) mRNA abundance of , and and 250 mg/kg LFP upregulated ( < 0.05) the expression of β, , and α α in the thymus. In conclusion, LFP supplementation improved the immune function of piglets by regulating the activity of serum biochemical enzymes, improving the antioxidant capacity, and alleviating inflammation of immune organs. This study indicated that LFP is potential alternative protection against early weaned stress in piglets.
断奶常诱发仔猪氧化应激和炎症反应。本研究探讨日粮添加甘草黄酮粉（LFP）对断奶仔猪抗氧化能力和免疫力的影响。具体而言，将96头长白×大白×杜洛克（DLY）断奶仔猪随机分为4组，每组6个重复（每个重复4头猪），分别饲喂添加0、50、150和250 mg/kg LFP的日粮。试验持续5周。结果表明，日粮添加LFP能有效提高肝脏指数（<0.05）。此外，日粮添加LFP可降低血清天冬氨酸转氨酶活性（<0.01）。饲喂50 mg/kg LFP的仔猪血清总胆固醇和高密度脂蛋白胆固醇含量降低（<0.05），血清碱性磷酸酶活性升高（<0.01）。同样，添加150 mg/kg LFP可提高血清总抗氧化能力（T-AOC）活性（<0.01），添加150和250 mg/kg LFP的日粮可提高脾脏T-AOC活性（<0.01）。此外，添加150 mg/kg LFP可提高仔猪血清IgG含量（<0.05）。另外，与对照组相比，日粮添加250 mg/kg LFP可上调脾脏中（β和）的mRNA丰度（<0.05）。同时，日粮添加150和250 mg/kg LFP可下调、的mRNA丰度（<0.05），250 mg/kg LFP可上调胸腺中β、、αα的表达（<0.05）。总之，添加LFP通过调节血清生化酶活性、提高抗氧化能力和减轻免疫器官炎症来改善仔猪免疫功能。本研究表明，LFP是预防仔猪早期断奶应激的潜在替代保护剂。
