Luo Junqiu, Wu Xiu, Chen Daiwen, Yu Bing, He Jun
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, People's Republic of China.
J Anim Sci Biotechnol. 2024 Oct 7;15(1):134. doi: 10.1186/s40104-024-01086-5.
Oxidative stress significantly impacts growth performance and liver function in piglets. Ferulic acid (FA) works as an antioxidant, however, the role and mechanism of FA in the regulation of diquat-induced oxidative stress in piglets are less known. This study was designed to investigate the effects of FA on growth performance and antioxidant capacity in piglets with diquat challenge.
Thirty-two healthy DLY (Duroc × Landrace × Yorkshire) piglets (13.24 ± 0.19 kg) were randomly divided into one of two diets including 0 or 4 g/kg FA for 14 d. On d 15, all pigs were intraperitoneally injected diquat or sterile saline.
Dietary supplementation with ferulic acid (FA) significantly improved the average daily gain (ADG) and decreased feed-gain ratio (F/G) of piglets. Here, dietary FA supplementation reduced serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities in diquat challenged piglets. Furthermore, diquat infusion increased reactive oxygen radicals (ROS) level in liver, decreased the activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) and increased malondialdehyde (MDA) content in the liver and serum. Supplementation with FA significantly increased T-AOC and T-SOD activities and decreased MDA and ROS levels. FA down-regulated gene and protein expression of Keap1, and up-regulated protein expression of Nrf2 and HO-1 in the liver of piglets with diquat challenge. Importantly, diquat challenge increased the ratio of late apoptosis, increased serum levels of IL-1β, IL-18 and lactate dehydrogenase (LDH), and up-regulated pyroptosis-related genes in the liver. FA supplementation reduced the ratio of late apoptosis and down-regulated mRNA expression of Caspase-1. Accordingly, FA addition reduced concentration of IL-1β, IL-18, and LDH under diquat challenge.
Diquat-induced oxidative stress reduced growth performance and impaired liver function in piglets. Dietary FA supplementation enhanced the antioxidant capacity and reduced the degree of hepatocyte pyroptosis, thereby alleviating the oxidative damage in the liver and mitigating the impact of diquat on growth performance of piglets.
氧化应激对仔猪的生长性能和肝功能有显著影响。阿魏酸（FA）作为一种抗氧化剂，然而，FA在调节仔猪敌草快诱导的氧化应激中的作用和机制尚不清楚。本研究旨在探讨FA对受敌草快攻击的仔猪生长性能和抗氧化能力的影响。
将32头健康的DLY（杜洛克×长白×约克夏）仔猪（13.24±0.19 kg）随机分为两种日粮组之一，分别含0或4 g/kg FA，持续14天。在第15天，所有仔猪腹腔注射敌草快或无菌生理盐水。
日粮中添加阿魏酸（FA）显著提高了仔猪的平均日增重（ADG），并降低了料重比（F/G）。在此，日粮中添加FA降低了受敌草快攻击仔猪的血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）活性。此外，注射敌草快增加了肝脏中的活性氧自由基（ROS）水平，降低了总超氧化物歧化酶（T-SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性、总抗氧化能力（T-AOC），并增加了肝脏和血清中的丙二醛（MDA）含量。添加FA显著增加了T-AOC和T-SOD活性，并降低了MDA和ROS水平。FA下调了受敌草快攻击仔猪肝脏中Keap1的基因和蛋白表达，并上调了Nrf2和HO-1的蛋白表达。重要的是，敌草快攻击增加了晚期凋亡比例，增加了血清白细胞介素-1β（IL-1β）、白细胞介素-18（IL-18）和乳酸脱氢酶（LDH）水平，并上调了肝脏中焦亡相关基因。添加FA降低了晚期凋亡比例，并下调了Caspase-1的mRNA表达。因此，添加FA降低了敌草快攻击下IL-1β、IL-18和LDH的浓度。
敌草快诱导的氧化应激降低了仔猪的生长性能并损害了肝功能。日粮中添加FA增强了抗氧化能力，降低了肝细胞焦亡程度，从而减轻了肝脏中的氧化损伤，并减轻了敌草快对仔猪生长性能的影响。
