Laitinen J, Löppönen R, Merenmies J, Rauvala H
FEBS Lett. 1987 Jun 8;217(1):94-100. doi: 10.1016/0014-5793(87)81250-4.
Binding of laminin to glycolipids of neuronal membranes was studied with a thin-layer chromatography overlay assay. The major brain ganglioside GD1A was the main binding component, when chromatograms containing the same molar amount of the different brain gangliosides and the brain sulfatide were incubated with laminin at physiological ionic strength. The possible role of laminin binding to brain gangliosides in laminin-neuron interactions was studied with adhesion assays. It was found that binding of rat brain neurons to laminin is blocked by 10-40 microM brain gangliosides but not by sulfatide. The inhibition by the gangliosides is suggested to be due to competition with the cell surface interaction sites of laminin and not to binding of the gangliosides to the cells. Our findings support the idea that the adhesive and neurite-promoting effect of laminin is dependent on its interaction with gangliosides at the neuronal cell surfaces.
采用薄层色谱覆盖分析法研究了层粘连蛋白与神经细胞膜糖脂的结合情况。当含有相同摩尔量的不同脑苷脂和脑硫脂的色谱图在生理离子强度下与层粘连蛋白一起孵育时，主要的脑苷脂GD1A是主要的结合成分。通过黏附试验研究了层粘连蛋白与脑苷脂结合在层粘连蛋白-神经元相互作用中的可能作用。发现大鼠脑神经元与层粘连蛋白的结合被10 - 40微摩尔的脑苷脂阻断，但不被脑硫脂阻断。推测脑苷脂的抑制作用是由于与层粘连蛋白的细胞表面相互作用位点竞争，而不是脑苷脂与细胞的结合。我们的研究结果支持这样一种观点，即层粘连蛋白的黏附作用和促进神经突生长的作用取决于其与神经元细胞表面脑苷脂的相互作用。
