加州电鳐11S型乙酰胆碱酯酶构象的拉曼光谱研究

Raman spectroscopic study on the conformation of 11 S form acetylcholinesterase from Torpedo californica.

作者信息

Aslanian D, Gróf P, Négrerie M, Balkanski M, Taylor P

出版信息

FEBS Lett. 1987 Jul 13;219(1):202-6. doi: 10.1016/0014-5793(87)81216-4.

Abstract

Vibrational Raman spectroscopy has been used to study the conformation of the 11 S form of acetylcholinesterase from Torpedo californica. Secondary structure analysis by the method of Williams [(1983) J. Mol. Biol. 166, 581-603] shows 49% alpha-helical structure, 23% beta-sheets, 11% turns and 15% undefined structure. Secondary structure estimates obtained for this enzyme by Raman spectroscopy and circular dichroism have been analyzed.

摘要

振动拉曼光谱已被用于研究来自电鳐的乙酰胆碱酯酶11S形式的构象。通过Williams方法[(1983) J. Mol. Biol. 166, 581 - 603]进行的二级结构分析表明，其具有49%的α-螺旋结构、23%的β-折叠、11%的转角和15%的未定义结构。已对通过拉曼光谱和圆二色性获得的该酶二级结构估计值进行了分析。