Meng Shengxi, Li Shaopeng, Chen Huize, Deng Chujun, Meng Zeyu, Wang Yimo
Department of Traditional Chinese Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
Shanghai Sensichip Infotech, Shanghai 200331, China.
Evid Based Complement Alternat Med. 2022 Aug 2;2022:5912396. doi: 10.1155/2022/5912396. eCollection 2022.
Alzheimer's disease (AD) seriously damages elders' social and daily abilities around the world. Traditional Chinese medicine (TCM), a rich drug resource bank, could help research AD. In order to explore the role of TCM in AD treatment, 86 AD patients were recruited from the hospital, then treated with Hengqing II prescription and donepezil hydrochloride. The cognitive and serum lipid levels were investigated before and after treatment. The patient's urine was collected after three months of treatment. Metabolites in the urine samples were extracted with methanol and detected on the UHPLC-MS platform. Results proved that Hengqing II can improve cognitive levels and reduce the levels of Hcy, D-D, FIB, Apo B, TC, and LDL-C compared with donepezil hydrochloride ( < 0.05). The results of multivariate statistical analysis revealed that the metabolism of HQII was significantly different compared with Control groups. A total of 66 differential metabolites were found in this comparison (50 were down-regulated and 16 were up-regulated). Four amino acid pathways and one linoleic acid pathway were found through these metabolites. After receiver operating characteristic analysis, it was suggested that palmitic acid, palmitoleic acid, linoleic acid, oleic acid, SAH, and methionine can be used as biomarkers for treating AD, while the effects of daidzein, genistein, and naringenin on the treatment of AD need to be further studied.
阿尔茨海默病（AD）在全球范围内严重损害老年人的社交和日常生活能力。中药（TCM）作为一个丰富的药物资源库，有助于AD的研究。为了探讨中药在AD治疗中的作用，从医院招募了86例AD患者，然后用恒清II方和盐酸多奈哌齐进行治疗。治疗前后对认知和血脂水平进行了调查。治疗三个月后收集患者尿液。尿液样本中的代谢物用甲醇提取，并在超高效液相色谱-质谱平台上进行检测。结果证明，与盐酸多奈哌齐相比，恒清II能提高认知水平，降低同型半胱氨酸、D-二聚体、纤维蛋白原、载脂蛋白B、总胆固醇和低密度脂蛋白胆固醇水平（<0.05）。多变量统计分析结果显示，与对照组相比，恒清II的代谢存在显著差异。在该比较中总共发现了66种差异代谢物（50种下调，16种上调）。通过这些代谢物发现了四条氨基酸途径和一条亚油酸途径。经过受试者工作特征分析，提示棕榈酸、棕榈油酸、亚油酸、油酸、S-腺苷高半胱氨酸和蛋氨酸可作为治疗AD的生物标志物，而大豆苷元、染料木黄酮和柚皮苷对AD治疗的作用有待进一步研究。
