Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba 305-8566, Japan.
S&B Foods Inc., #605 MITSUI LINK-Lab Shinkiba1 Shinkiba 2-3-8, Koto-ku, Tokyo 136-0082, Japan.
Food Funct. 2022 Sep 22;13(18):9407-9418. doi: 10.1039/d2fo01036d.
We showed that an ethanol extract from can shorten the circadian rhythm at the cellular level and that this activity was due to hydroxy-β-sanshool, a secondary metabolite in this plant. An ethanol extract of was repeatedly fractionated using solid phase extraction and reverse-phase HPLC, then the circadian rhythms of cells to which the fractions were loaded were monitored using real-time reporter gene assays. We purified one HPLC peak and identified it as hydroxy-β-sanshool using liquid chromatography (LC)-precision-mass spectrometry (MS). This compound shortened the period of and at the cellular level. Incubation cells for 24 h with hydroxy-β-sanshool resulted in upregulated promoter activity. Hydroxy-β-sanshool also dose-dependently upregulated expression of the clock genes , , and and the clock-controlled oxidative stress responsive genes and .
我们表明， 提取物可缩短细胞水平的昼夜节律，而这种活性归因于该植物中的次级代谢产物羟基-β-山椒素。 使用固相萃取和反相 HPLC 反复对 的乙醇提取物进行分级，然后使用实时报告基因测定法监测加载了这些级分的细胞的昼夜节律。我们使用液相色谱（LC）-精密质谱（MS）对一个 HPLC 峰进行了纯化，并将其鉴定为羟基-β-山椒素。该化合物在细胞水平上缩短了 和 的周期。用羟基-β-山椒素孵育细胞 24 小时会导致 启动子活性上调。羟基-β-山椒素还剂量依赖性地上调时钟基因 、 、 和 以及时钟控制的氧化应激反应基因 和 的表达。
