Spiro M J
Diabetologia. 1987 Apr;30(4):259-67. doi: 10.1007/BF00270425.
The incorporation of [35S]sulfate into heart proteoglycans has been studied in normal and alloxan-diabetic rats by perfusion and in vivo administration of the isotope; in the latter situation, comparison was also made of radiolabeled sulfate utilization by several other tissues (kidney, liver, lung, muscle, testes and skin). The radiolabeled products were characterized by sodium dodecylsulfate polyacrylamide gel electrophoresis and anion exchange chromatography, as well as by sizing of the glycosaminoglycan chains by gel filtration both before and after nitrous acid treatment. The most prominent band observed in the heart guanidine extract by electrophoresis had a molecular weight of 85,000 and minor components (Mr = 360,000 and 170,000) were also detected; approximately 20% of the proteoglycan associated [35S]sulfate was present in heparan sulfate chains. After perfusion the pattern, as well as the amount of radioactivity recovered from the diabetic heart, was similar to the normal heart. In contrast, after intraperitoneal injection of the [35S]sulfate, a substantial reduction in incorporation was found not only in heart but in several other tissues studied, although no qualitative differences were noted in the macromolecules formed by the two groups of animals. Measurement of the serum sulfate concentration indicated that the level in the alloxan-diabetic rat (1.23 mmol/l) was significantly less (p less than 0.01) than that of the normal rat (1.67 mmol/l).(ABSTRACT TRUNCATED AT 250 WORDS)
通过灌注以及体内给予同位素的方式，对正常大鼠和四氧嘧啶诱导的糖尿病大鼠心脏蛋白聚糖中[35S]硫酸盐的掺入情况进行了研究；在体内给予同位素的情况下，还比较了其他几种组织（肾脏、肝脏、肺、肌肉、睾丸和皮肤）对放射性标记硫酸盐的利用情况。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳、阴离子交换色谱对放射性标记产物进行了表征，同时在亚硝酸处理前后，通过凝胶过滤对糖胺聚糖链的大小进行了测定。通过电泳在心脏胍提取物中观察到的最突出条带的分子量为85,000，还检测到了次要成分（Mr = 360,000和170,000）；约20%的与蛋白聚糖相关的[35S]硫酸盐存在于硫酸乙酰肝素链中。灌注后，糖尿病心脏的放射性模式以及回收的放射性活度与正常心脏相似。相比之下，腹腔注射[35S]硫酸盐后，不仅在心脏，而且在所研究的其他几种组织中，掺入量都大幅降低，尽管两组动物形成的大分子没有质的差异。血清硫酸盐浓度的测定表明，四氧嘧啶诱导的糖尿病大鼠的水平（1.23 mmol/l）明显低于正常大鼠（1.67 mmol/l）（p < 0.01）。（摘要截短于250字）
