Key Laboratory for Special Functional Materials of the Ministry of Education, Henan University, Kaifeng 475004, P. R. China.
School of Physics and Electronics, Henan University, Kaifeng 475004, P. R. China.
Anal Chem. 2022 Aug 23;94(33):11557-11563. doi: 10.1021/acs.analchem.2c01793. Epub 2022 Aug 12.
Surface-enhanced Raman scattering (SERS)-based immunoassay on encoded beads is highly attractive with the advantages of ultrasensitivity, multiplex and high throughput. However, it was a great challenge to screen out in-focus signals of the immunoconjugated SERS nanoprobes on spherical bead conveniently. Here, periodic SERS-encoded magnetic beads (PSE-MBs) were developed through droplet optofluidic technique by using monodisperse SERS-encoded magnetic nanospheres as building blocks. The designed PSE-MBs not only exhibit huge coding capacity, but also provide the strongest and reproducible SERS coding signals as "in-focus beacons". When PSE-MBs are used as capture carriers in SERS-based immunoassay, both multiple target analytes and in-focus signals of SERS nanoprobes could be easily identified according to the collected SERS coding signals. Thus, reliable quantitative analysis of multiple target analytes could be conveniently achieved by such detection protocol. Additionally, the magnetic ingredient in PSE-MBs made the operation easily during the bioassay. The multiple advantages of PSE-MBs including large coding capacity, in-focus beacons and magnetic operation endorse them to be robust capture carriers in reliable quantitative SERS-based multiplex immunoassay.
基于表面增强拉曼散射 (SERS) 的编码珠上免疫分析具有超灵敏、多重和高通量的优点。然而，方便地筛选出免疫共轭 SERS 纳米探针在球形珠上的聚焦信号仍然是一个巨大的挑战。在这里，通过液滴光流技术，使用单分散 SERS 编码磁性纳米球作为构建块，开发了周期性 SERS 编码磁性珠 (PSE-MB)。设计的 PSE-MB 不仅具有巨大的编码容量，而且提供最强和可重复的 SERS 编码信号作为“聚焦信标”。当 PSE-MB 用作 SERS 免疫分析中的捕获载体时，根据收集的 SERS 编码信号，可以轻松识别多个目标分析物和 SERS 纳米探针的聚焦信号。因此，通过这种检测方案可以方便地实现对多个目标分析物的可靠定量分析。此外，PSE-MB 中的磁性成分使生物测定过程中的操作变得容易。PSE-MB 的多种优势，包括大容量编码、聚焦信标和磁性操作，使它们成为可靠定量 SERS 多重免疫分析中的强大捕获载体。
