分析 MSTN 基因突变猪二代杂交后代的肉质颜色、嫩度和脂肪酸组成。
Analysis of meat color, meat tenderness and fatty acid composition of meat in second filial hybrid offspring of MSTN mutant pigs.
机构信息
Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji 133002, China; Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji 133002, China.
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji 133002, China.
出版信息
Meat Sci. 2022 Nov;193:108929. doi: 10.1016/j.meatsci.2022.108929. Epub 2022 Jul 29.
The objective of this study was to assess the meat quality in second filial hybrid offspring of MSTN cloning boars × Chinese Bama sows with initial mean body weight of 90.52 ± 0.68 kg. Compared with wild-type pigs, the feed utilization rate of MSTN pigs showed an increasing trend (adjusted P = 0.06), loin eye area of MSTN and MSTN pigs increased (adjusted P < 0.01) and thickness of subcutaneous fat decreased (adjusted P < 0.01), the proportion of polyunsaturated fatty acids in meat of MSTN pigs increased (adjusted P < 0.05). By means of histochemical staining, immunofluorescence, scanning electron microscopy, qRT-PCR and WB technologies, it was verified that the decreased meat color score of MSTN pigs was related to the increase of type IIB muscle fiber, and the increased pork tenderness was related to the decrease of collagen content. Overall, this research provides reference for the further utilization of MSTN gene mutant pigs.
本研究旨在评估 MSTN 基因敲除公猪×中国巴马小型猪杂交二代子猪的肉质，其初始平均体重为 90.52±0.68kg。与野生型猪相比，MSTN 猪的饲料利用率呈上升趋势（调整后 P=0.06），MSTN 猪和 MSTN 猪的腰眼面积增加（调整后 P<0.01），皮下脂肪厚度降低（调整后 P<0.01），肉中多不饱和脂肪酸的比例增加（调整后 P<0.05）。通过组织化学染色、免疫荧光、扫描电子显微镜、qRT-PCR 和 WB 技术，验证了 MSTN 猪的肉色评分降低与 IIB 型肌纤维增加有关，猪肉嫩度增加与胶原蛋白含量降低有关。总之，本研究为进一步利用 MSTN 基因突变猪提供了参考。
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