Luo Shan, Liu Yuanqing, Bu Lige, Wang Dezheng, Wen Zhaoyu, Yang Yuqing, Xu Yanan, Wu Di, Li Guangpeng, Yang Lei
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China.
Animals (Basel). 2024 Dec 27;15(1):47. doi: 10.3390/ani15010047.
Myostatin (MSTN) serves as a negative regulatory factor for muscle development. A reduction in MSTN gene expression can enhance muscle mass and increase meat production. However, whether it will impact meat quality traits remains one of the major concerns in the cattle breeding industry. To explore the meat quality traits of MSTN gene-edited cattle, this study compared the meat quality traits of three MSTN gene-edited cattle breeds with those of non-gene-edited cattle, including Luxi, Angus, and Mongolian cattle, and further conducted an analysis in combination with metabolomics. We found that MSTN gene-edited cattle were superior to non-gene-edited cattle in terms of meat pH, shear force, and intramuscular fat content. However, no significant differences were observed in water-holding capacity, water content, and protein content of the meat. Metabolomics analysis revealed three upregulated differential metabolites common to MSTN gene-edited and non-gene-edited cattle across three breeds, namely lactoyl-valine, 3-phenylactic acid, and lactoyl-methionine. Some studies have indicated that these metabolites can improve the meat's flavor. In this study, we compared the meat quality traits and metabolomics data between MSTN gene-edited and non-gene-edited cattle, and found that the meat quality of MSTN gene-edited cattle was superior to that of non-gene-edited cattle.
肌肉生长抑制素（MSTN）是肌肉发育的负调控因子。MSTN基因表达的降低可增加肌肉量并提高产肉量。然而，它是否会影响肉质性状仍是肉牛育种行业的主要关注点之一。为探究MSTN基因编辑牛的肉质性状，本研究比较了三个MSTN基因编辑牛品种（包括鲁西牛、安格斯牛和蒙古牛）与非基因编辑牛的肉质性状，并结合代谢组学进行了分析。我们发现，MSTN基因编辑牛在肉的pH值、剪切力和肌内脂肪含量方面优于非基因编辑牛。然而，在肉的持水力、含水量和蛋白质含量方面未观察到显著差异。代谢组学分析揭示了三个品种的MSTN基因编辑牛和非基因编辑牛共有的三种上调差异代谢物，即乳酰缬氨酸、3-苯乳酸和乳酰甲硫氨酸。一些研究表明，这些代谢物可改善肉的风味。在本研究中，我们比较了MSTN基因编辑牛和非基因编辑牛的肉质性状和代谢组学数据，发现MSTN基因编辑牛的肉质优于非基因编辑牛。
