Rosebrough R W, Steele N C, McMurtry J P, Richards M P, Mitchell A D, Calvert C C
Growth. 1986 Winter;50(4):461-71.
Experiments were conducted with dwarf (dw) and normal lines of chickens to determine the effect of sex, diet and line on lipogenesis in the 28-day-old chick. The chicks were fed diets containing 12, 18, 23 and 30% protein. In the first experiment, in vitro lipogenesis (incorporation of [2-14C] sodium acetate into hepatic fatty acids) as well as growth from 7 to 28 days of age were determined in males and females of both lines. In the second experiment, only males and females of the dwarf line were fed to determine the relative contribution of acetate and pyruvate to in vitro lipogenesis (incorporation of either [2-14C] sodium acetate or [2-14C] pyruvate into hepatic fatty acids). Chicks of the dwarf line were smaller (P less than .01) than were those of the normal line. Females of both lines were smaller (P less than .01) than males. In vitro lipogenesis was lower (P less than .01) in the dwarf line; however, the rate for both sexes within a given line was equal. An increase in the dietary protein decreased (P less than .05) in vitro lipogenesis in both lines. The use of pyruvate as an in vitro precursor indicated that the regulation of lipid and carbohydrate metabolism may be an integrated process involving pyruvate carboxylation and subsequent flux of pyruvate carbon into either glucose or fatty acids. Based on the data presented, there is no evidence to assume, that the dwarf gene per se influences lipogenesis.
用矮小型（dw）和正常品系的鸡进行实验，以确定性别、日粮和品系对28日龄雏鸡脂肪生成的影响。给雏鸡饲喂含12%、18%、23%和30%蛋白质的日粮。在第一个实验中，测定了两个品系雄性和雌性雏鸡7至28日龄期间的体外脂肪生成（[2-14C]醋酸钠掺入肝脏脂肪酸的情况）以及生长情况。在第二个实验中，仅给矮小型品系的雄性和雌性雏鸡饲喂，以确定醋酸盐和丙酮酸对体外脂肪生成（[2-14C]醋酸钠或[2-14C]丙酮酸掺入肝脏脂肪酸的情况）的相对贡献。矮小型品系的雏鸡比正常品系的雏鸡小（P<0.01）。两个品系的雌性雏鸡都比雄性雏鸡小（P<0.01）。矮小型品系的体外脂肪生成较低（P<0.01）；然而，给定品系内两性的脂肪生成率是相等的。日粮蛋白质增加会使两个品系的体外脂肪生成减少（P<0.05）。使用丙酮酸作为体外前体表明，脂质和碳水化合物代谢的调节可能是一个涉及丙酮酸羧化以及随后丙酮酸碳流入葡萄糖或脂肪酸的整合过程。根据所提供的数据，没有证据表明矮小型基因本身会影响脂肪生成。
