Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringa, Parana, Brazil.
Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringa, Parana, Brazil.
Nutrition. 2022 Nov-Dec;103-104:111776. doi: 10.1016/j.nut.2022.111776. Epub 2022 Jun 17.
Maternal circadian eating time and frequency are associated with altered glucose metabolism during pregnancy in humans. Research on long maternal fasting intervals is inconclusive, and little is known about the effect of maternal time feeding on offspring health. Therefore, the aim of the present study is to determine whether maternal time feeding influences the metabolic status of both male and female offspring.
Pregnant rats were provided ad libitum access to chow diet or fed during either the light phase (LP) or dark phase (DP) during embryonic development. At the age of 150 days, glucose tolerance, lipid concentrations, and insulin secretion were determined in adult male and female offspring.
Both male and female offspring of LP and DP dams exhibited alterations in the lipid profile, and female offspring were glucose intolerant. Glucose-stimulated insulin secretion decreased in male and female offspring of LP and DP dams. Acetylcholine increased insulin secretion in male and female offspring. Islets from male and female offspring of DP dams exhibited less pronounced inhibition of insulin secretion by epinephrine, suggesting alterations in the cholinergic and adrenergic pathways in these animals.
Our data suggest that a time-restricted feeding regimen during embryonic development could program rat offspring for metabolic dysfunction during adulthood.
母体的昼夜进食时间和频率与人类妊娠期间葡萄糖代谢的改变有关。关于母体长时间禁食的研究尚无定论，而且对于母体时间喂养对子代健康的影响知之甚少。因此，本研究旨在确定母体时间喂养是否会影响雄性和雌性后代的代谢状态。
给予怀孕大鼠自由摄取标准食物或在胚胎发育期间的亮期（LP）或暗期（DP）进行喂养。在 150 天大时，测定成年雄性和雌性后代的葡萄糖耐量、脂质浓度和胰岛素分泌。
LP 和 DP 组母鼠的雄性和雌性后代的脂质谱均发生改变，且雌性后代出现葡萄糖不耐受。LP 和 DP 组母鼠的雄性和雌性后代的葡萄糖刺激胰岛素分泌减少。乙酰胆碱增加雄性和雌性后代的胰岛素分泌。来自 DP 组母鼠的雄性和雌性后代的胰岛对肾上腺素抑制胰岛素分泌的作用减弱，提示这些动物的胆碱能和肾上腺素能途径发生改变。
我们的数据表明，胚胎发育期间的限时喂养方案可能会导致成年后大鼠后代出现代谢功能障碍。
