柞蚕多核衣壳核型多角体病毒（AnpeNPV）凋亡抑制蛋白1（IAP1）在病毒复制和包涵体产生中的功能分析

Functional analyses of inhibitor of apoptosis protein 1 (IAP1) of Antheraea pernyi multinucleocapsid nucleopolyhedrovirus (AnpeNPV) in viral replication and occlusion body production.

作者信息

Zhao Zhenjun, Yue Dongmei, Ye Bo, Li Peipei, Li Wenli, Wang Linmei, Zhang Bo, Fan Qi

机构信息

Liaoning Ocean and Fisheries Science Research Institute, Liaoning Academy of Agricultural Sciences, Dalian 116023, China.

School of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin 124211, China.

出版信息

J Invertebr Pathol. 2022 Oct;194:107816. doi: 10.1016/j.jip.2022.107816. Epub 2022 Aug 11.

DOI:10.1016/j.jip.2022.107816

PMID:35964678

Abstract

Inhibitor of apoptosis protein 1 (IAP1) of Antheraea pernyi multinucleocapsid nucleopolyhedrovirus (AnpeNPV) belongs to the baculovirus IAP1 type. The function of AnpeNPV-IAP1 in viral replication and occlusion body (OB) production remains unknown. In this study, we demonstrated that AnpeNPV-iap1 is a late gene. AnpeNPV-IAP1 mainly localizes to the nuclear ring zone and exhibits dynamic distribution in the cytoplasm and the virogenic stroma during AnpeNPV infection. AnpeNPV-IAP1 impacted the expression of a variety of viral genes at the very late phase of infection in Tn-Hi5 cells. The deletion of AnpeNPV-iap1 caused decreased expression levels of polyhedrin, morphological changes to OBs and reduced OB production in A. pernyi pupae, along with a lengthening of the lethal time of A. pernyi larvae. These results suggest that AnpeNPV-iap1 is involved in regulating viral gene expression, OB production and morphogenesis in A. pernyi.

摘要

柞蚕多核衣壳核型多角体病毒（AnpeNPV）的凋亡抑制蛋白1（IAP1）属于杆状病毒IAP1类型。AnpeNPV - IAP1在病毒复制和包涵体（OB）产生中的功能尚不清楚。在本研究中，我们证明AnpeNPV - iap1是一个晚期基因。AnpeNPV - IAP1主要定位于核环区，在AnpeNPV感染期间在细胞质和病毒发生基质中表现出动态分布。AnpeNPV - IAP1在Tn - Hi5细胞感染的极晚期影响多种病毒基因的表达。AnpeNPV - iap1的缺失导致柞蚕蛹中多角体蛋白表达水平降低、OB形态变化以及OB产量减少，同时柞蚕幼虫的致死时间延长。这些结果表明AnpeNPV - iap1参与调控柞蚕中的病毒基因表达、OB产生和形态发生。