稳定、耐寒、温度敏感/条件致死嵌合肠道病毒 A71 和柯萨奇病毒 A16 的研制。
Development of stable, cold-adapted, temperature-sensitive/conditional lethal chimeric enterovirus A71 and coxsackievirus A16.
机构信息
Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604, Singapore.
Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604, Singapore.
出版信息
Virol Sin. 2022 Oct;37(5):769-773. doi: 10.1016/j.virs.2022.08.001. Epub 2022 Aug 11.
Abstract
• A stable EV-A71 virus vector was created to generate chimeric strains expressing capsid protein genes of EV-A71 C5 and CA16. • Phenotypic and genetic stability of the generated chimeric EV-A71 and CA16 were analyzed. • The amino acids at the cleavage site between VP1 and 2A is crucial for stability.
摘要
• 构建了稳定的 EV-A71 病毒载体，用于产生表达 EV-A71 C5 和 CA16 衣壳蛋白基因的嵌合株。• 分析了产生的嵌合 EV-A71 和 CA16 的表型和遗传稳定性。• VP1 和 2A 之间切割位点的氨基酸对于稳定性至关重要。
Zotero 插件