Roca Liana S, Adamopoulou Theodora, Nawada Suhas H, Schoenmakers Peter J
Van 't Hoff Institute for Molecular Sciences, Science Park 904, 1098 XH Amsterdam, The Netherlands.
Chromatographia. 2022;85(8):783-793. doi: 10.1007/s10337-022-04156-w. Epub 2022 Jun 22.
Microfluidic devices for comprehensive three-dimensional spatial liquid chromatography will ultimately require a body of stationary phase with multiple in- and outlets. In the present work, 3D printing with a transparent polymer resin was used to create a simplified device that can be seen as a unit cell for an eventual three-dimensional separation system. Complete packing of the device with 5-μm C18 particles was achieved, with reasonable permeability. The packing process could be elegantly monitored from the pressure profile, which implies that optical transparency may not be required for future devices. The effluent flow was different for each of the four outlets of the device, but all flows were highly repeatable, suggesting that correction for flow-rate variations is possible. The investigation into flow patterns through the device was supported by computational-fluid-dynamics simulations. A proof-of-principle separation of four standard peptides is described, with mass-spectrometric detection for each of the four channels separately.
The online version contains supplementary material available at 10.1007/s10337-022-04156-w.
用于全面三维空间液相色谱的微流控装置最终将需要一个具有多个进出口的固定相主体。在本工作中，使用透明聚合物树脂进行3D打印来制造一个简化装置，该装置可被视为最终三维分离系统的一个单元。用5μm的C18颗粒对该装置进行了完全填充，具有合理的渗透率。可以从压力曲线优雅地监测填充过程，这意味着未来的装置可能不需要光学透明性。该装置的四个出口的流出物流量各不相同，但所有流量都具有高度可重复性，这表明可以对流速变化进行校正。通过计算流体动力学模拟支持了对通过该装置的流动模式的研究。描述了四种标准肽的原理验证分离，并分别对四个通道中的每一个进行了质谱检测。
在线版本包含可在10.1007/s10337-022-04156-w获取的补充材料。
