GLIPR1调节胶质瘤细胞中的TIMP1-CD63-ITGB1-AKT信号通路，并诱导星形胶质瘤发生恶性转化。

GLIPR1 regulates the TIMP1-CD63-ITGB1-AKT signaling pathway in glioma cells and induces malignant transformation of astroglioma.

作者信息

Li Feng, Zhang Weifeng, Wang Ming, Jia Pifeng

机构信息

Department of Neurosurgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

出版信息

Transl Cancer Res. 2022 Jul;11(7):2205-2216. doi: 10.21037/tcr-21-2413.

DOI:10.21037/tcr-21-2413

PMID:35966296

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9372201/

Abstract

BACKGROUND

Astrocytoma (ACM) is characterized by high recurrence rate, high mortality, and extremely poor clinical prognosis. The new diagnostic and prognostic tumor markers related to ACM was found to improve the diagnosis rate and reduce the poor prognosis.

METHODS

The activity of SHC-44 and SW1783 cells under the regulation of glioma pathogenesis-related protein 1 (GLIPR1) was investigated by CCK8 analysis. The effect of GLIPR1 on the proliferation of SHC-44 and SW1783 cells was analyzed by cell colony-forming experiment. The migration of SHC-44 and SW1783 cells under the regulation of GLIPR1 was analyzed by transwell assay. The effects of GLIPR1 on the invasion and migration of SHC-44 and SW1783 cells were analyzed by cell scratch test and transwell assay. Immunofluorescence and Co-IP assays were employed to analyze the expression characteristics of GLIPR1 and CD63 proteins. The effect of GLIPR1 on the protein expression of GLIPR1, TIMP1, CD63, ITGB1, and AKT in SHC-44 and SW1783 cells was analyzed by western blot. The effect of anti-AKT on the protein expression of GLIPR1, TIMP1, CD63, ITGB1, and AKT in SHC-44 and SW1783 cells was performed by western blot.

RESULTS

The outcomes revealed that GLIPR1 could enhance the activity, proliferation, migration, and invasion of ACM cells, which might be associated with the activation of the TIMP1-CD63-ITGB1-AKT signaling pathway.

CONCLUSIONS

Taken together, GLIPR1 might be a potential target for the prevention or management of ACM in the clinic.

摘要

背景

星形细胞瘤（ACM）具有高复发率、高死亡率和极差的临床预后等特征。发现与ACM相关的新型诊断和预后肿瘤标志物可提高诊断率并改善不良预后。

方法

通过CCK8分析研究神经胶质瘤发病相关蛋白1（GLIPR1）调控下SHC - 44和SW1783细胞的活性。通过细胞集落形成实验分析GLIPR1对SHC - 44和SW1783细胞增殖的影响。通过Transwell实验分析GLIPR1调控下SHC - 44和SW1783细胞的迁移情况。通过细胞划痕实验和Transwell实验分析GLIPR1对SHC - 44和SW1783细胞侵袭和迁移的影响。采用免疫荧光和免疫共沉淀实验分析GLIPR1和CD63蛋白的表达特征。通过蛋白质印迹法分析GLIPR1对SHC - 44和SW1783细胞中GLIPR1、TIMP1、CD63、ITGB1和AKT蛋白表达的影响。通过蛋白质印迹法检测抗AKT对SHC - 44和SW1783细胞中GLIPR1、TIMP1、CD63、ITGB1和AKT蛋白表达的作用。