Concistrè Antonio, Petramala Luigi, Circosta Francesco, Romagnoli Priscilla, Soldini Maurizio, Bucci Marco, De Cesare Domenico, Cavallaro Giuseppe, De Toma Giorgio, Cipollone Francesco, Letizia Claudio
Department of Clinical, Internal Medicine, Anesthesiology and Cardiovascular Sciences, "Sapienza" University of Rome, Rome, Italy.
Department of Translational and Precision Medicine, "Sapienza" University of Rome, Rome, Italy.
Front Cardiovasc Med. 2022 Jul 28;9:930959. doi: 10.3389/fcvm.2022.930959. eCollection 2022.
Primary aldosteronism (PA) is characterized by several metabolic changes such as insulin resistance, metabolic syndrome, and adipose tissue (AT) inflammation. Mi(cro)RNAs (miRNAs) are a class of non-coding small RNA molecules known to be critical regulators in several cellular processes associated with AT dysfunction. The aim of this study was to evaluate the expression of some miRNAs in visceral and subcutaneous AT in patients undergoing adrenalectomy for aldosterone-secreting adrenal adenoma (APA) compared to the samples of AT obtained in patients undergoing adrenalectomy for non-functioning adrenal mass (NFA).
The quantitative expression of selected miRNA using real-time PCR was analyzed in surrounding adrenal neoplasia, peri-renal, and subcutaneous AT samples of 16 patients with adrenalectomy (11 patients with APA and 5 patients with NFA).
Real-time PCR cycles for miRNA-132, miRNA-143, and miRNA-221 in fat surrounding adrenal neoplasia and in peri-adrenal AT were significantly higher in APA than in patients with NFA. Unlike patients with NFA, miRNA-132, miRNA-143, miRNA-221, and miRNA-26b were less expressed in surrounding adrenal neoplasia AT compared to subcutaneous AT in patients with APA.
This study, conducted on tissue expression of miRNAs, highlights the possible pathophysiological role of some miRNAs in determining the metabolic alterations in patients with PA.
原发性醛固酮增多症（PA）的特征是存在多种代谢变化，如胰岛素抵抗、代谢综合征和脂肪组织（AT）炎症。微小RNA（miRNA）是一类非编码小RNA分子，已知在与AT功能障碍相关的多个细胞过程中起关键调节作用。本研究的目的是评估在因分泌醛固酮的肾上腺腺瘤（APA）而行肾上腺切除术的患者的内脏和皮下AT中某些miRNA的表达，并与因无功能肾上腺肿块（NFA）而行肾上腺切除术的患者所获得的AT样本进行比较。
对16例行肾上腺切除术的患者（11例APA患者和5例NFA患者）的肾上腺肿瘤周围、肾周和皮下AT样本，使用实时PCR分析所选miRNA的定量表达。
肾上腺肿瘤周围脂肪和肾上腺周围AT中，APA患者的miRNA-132、miRNA-143和miRNA-221的实时PCR循环数显著高于NFA患者。与NFA患者不同，APA患者肾上腺肿瘤周围AT中miRNA-132、miRNA-143、miRNA-221和miRNA-26b的表达低于皮下AT。
本研究对miRNA的组织表达进行了分析，突出了某些miRNA在PA患者代谢改变中可能的病理生理作用。
