Zhu Chengyi, Pan Lanlan, Yi Qiong, Wei Qiping
Ophthalmology Department, Beijing He Ping Li Hospital, Beijing 100013, China.
Ophthalmology Department, Beijing Oriental Hospital, University of Traditional Chinese Medicine, Beijing 100078, China.
J Ophthalmol. 2022 Aug 4;2022:1514244. doi: 10.1155/2022/1514244. eCollection 2022.
This study aimed to determine the expression levels of vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) in the aqueous humor of patients with macular edema (ME) caused by branch retinal vein occlusion (BRVO), as well as to investigate the relationship between the cytokines as mentioned earlier and best-corrected visual acuity (BCVA), ME, and the degree of ME from the molecular level.
In a prospective observational study, fluorescein fundus angiography (FFA) and optical coherence tomography (OCT) were used to classify 58 patients with non-ischemic BRVO-ME into three groups according to the degree of ME: 14-mild, 17-moderate, and 27-severe. The specific concentration of IL-6, VEGF, ICAM-1, and VCAM-1 in the aqueous humor was detected using the BD CSCanto™ II Flow Cytometer (US). Spearman or Pearson correlation analysis was used to test the correlation between the levels of BCVA and severity of ME and the expression levels of IL-6, VEGF, ICAM-1, and VCAM-1 in the aqueous humor.
According to the obtained data, BCVA did not correlate with the severity of ME, and these four cytokines expression levels in patients' aqueous humor ( > 0.05). Moreover, BCVA did not correlate with mild, moderate, or severe ME as well ( > 0.05). However, the levels of these four cytokines were correlated with the severity of the ME. These underlined cytokines were linked to the mild, moderate, and severe degrees of ME. VEGF was also significantly correlated ( > 0.8, < 0.0001) with the severity of ME.
This study suggests that the severity of ME in BRVO-ME patients is significantly correlated with the expression levels of IL-6, VEGF, ICAM-1, and VCAM-1 in the aqueous humor. Lowering the level of disease-associated cytokines may potentially reduce the degree of ME. Therefore, an in-depth study of the levels and the relationship may provide some evidence for the pathogenesis, treatment, and prevention of BRVO-ME.
本研究旨在测定视网膜分支静脉阻塞（BRVO）所致黄斑水肿（ME）患者房水中血管内皮生长因子（VEGF）、白细胞介素-6（IL-6）、细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的表达水平，并从分子水平探讨上述细胞因子与最佳矫正视力（BCVA）、ME及ME程度之间的关系。
在一项前瞻性观察研究中，采用荧光素眼底血管造影（FFA）和光学相干断层扫描（OCT），根据ME程度将58例非缺血性BRVO-ME患者分为三组：14例轻度、17例中度和27例重度。使用美国BD CSCanto™ II流式细胞仪检测房水中IL-6、VEGF、ICAM-1和VCAM-1的具体浓度。采用Spearman或Pearson相关分析来检验BCVA水平、ME严重程度与房水中IL-6、VEGF、ICAM-1和VCAM-1表达水平之间的相关性。
根据所得数据，BCVA与ME严重程度无关，患者房水中这四种细胞因子的表达水平也无相关性（>0.05）。此外，BCVA与轻度、中度或重度ME也均无相关性（>0.05）。然而，这四种细胞因子的水平与ME严重程度相关。这些相关细胞因子与轻度、中度和重度ME相关。VEGF与ME严重程度也显著相关（>0.8，<0.0001）。
本研究表明，BRVO-ME患者的ME严重程度与房水中IL-6、VEGF、ICAM-1和VCAM-1的表达水平显著相关。降低疾病相关细胞因子水平可能会降低ME程度。因此，深入研究这些水平及其关系可能为BRVO-ME的发病机制、治疗和预防提供一些依据。
Zotero 插件
