冷榨豆粕中产共轭亚油酸和二十碳五烯酸及其优化

Production and Optimization of Conjugated Linoleic and Eicosapentaenoic Acids by in Cold-Pressed Soybean Cake.

作者信息

Azari Samin Rafi, Hojjatoleslamy Mohammad, Mousavi Zeinab E, Kiani Hossein, Jalali Sayed Mohammad Ali

机构信息

Department of Food Science and Technology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Bioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

出版信息

Front Nutr. 2022 Jul 27;9:916728. doi: 10.3389/fnut.2022.916728. eCollection 2022.

DOI:10.3389/fnut.2022.916728

PMID:35967809

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9363755/

Abstract

BACKGROUND AND PURPOSE

In regard to the biosynthesis of conjugated linoleic acid (CLA) and eicosapentaenoic acid (EPA) by some bacteria, the objective of this study was to evaluate the efficiency of solid-state fermentation based on soybean pressed cake (SPC) to produce CLA and EPA by . The objective of this study was to evaluate the efficiency of solid-state fermentation based on SPC to produce CLA and EPA by .

METHODS

Process conditions including humidity, inoculation level, and temperature parameters were optimized by adopting the response surface methodology (RSM) method (response surface method) and the design expert software. Accordingly, a homogeneous SPC paste substrate at 60, 70, and 80% humidity was prepared with different inoculation levels at 30, 37, and 44°C to assess the strain behavior. The introduced SPC consisted of 60% humidity, 2% inoculation level at 37°C, and 60% humidity, and 4% inoculation level at 30 and 44°C; it also included 6% inoculation level at 37°C, 70% humidity at 2% inoculation level, at 30 and 44°C, and 4% inoculation level at 37°C. Also, SPC with 80% humidity at 2% and 4% inoculation levels, and at 30 and 44°C was obtained. To confirm the accuracy of the conditions, an experiment was conducted according to the defined requirements.

RESULTS

The results were compared with the predicted data, which showed a significant difference. Under optimized conditions, with an inoculation level of 4% on the SPC medium with 70% humidity and at 37°C, strains could yield 9, 11 linoleic and eicosapentaenoic at 0.18 and 0.39% of the total fatty acids.

CONCLUSION

So, the potential benefits of using SPC as an inexpensive substrate for the commercial production of CLA and EPA should be noted.

摘要

背景与目的

鉴于某些细菌可生物合成共轭亚油酸（CLA）和二十碳五烯酸（EPA），本研究的目的是评估基于豆粕（SPC）的固态发酵生产CLA和EPA的效率。本研究的目的是评估基于SPC的固态发酵生产CLA和EPA的效率。

方法

采用响应面法（RSM）和Design Expert软件对湿度、接种量和温度等工艺条件进行优化。据此，制备了湿度分别为60%、70%和80%的均匀SPC糊状物底物，并在30℃、37℃和44℃下设置不同接种量，以评估菌株行为。引入的SPC包括湿度为60%、接种量为2%、温度为37℃，湿度为60%、接种量为4%、温度为30℃和44℃；还包括接种量为6%、温度为37℃、湿度为70%、接种量为2%、温度为30℃和44℃，以及接种量为4%、温度为37℃。此外，还获得了湿度为80%、接种量为2%和4%、温度为30℃和44℃的SPC。为了确认条件的准确性，根据规定要求进行了实验。