de Paula Ailton Ferreira, Cruz Felipe de Paula Nogueira, Dinato Naiana Barbosa, de Andrade Paulo Henrique Marques, de Moraes Amanda Carolina Prado, Junior Waldomiro Barioni, Bernardi Alberto Carlos de Campos, Vigna Bianca Baccili Zanotto, Fávero Alessandra Pereira, Lacava Paulo Teixeira
Laboratory of Microbiology and Biomolecules, Department of Morphology and Pathology, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil.
Evolutionary Genetics and Molecular Biology Graduate Program, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil.
Front Plant Sci. 2022 Jul 28;13:884716. doi: 10.3389/fpls.2022.884716. eCollection 2022.
The genus belongs to the family Poaceae and has several species that are native to Brazil. The Germplasm Bank (GB) of the Brazilian Agricultural Research Corporation comprises approximately 450 accessions from 50 species. Among these accessions, (BGP 308) has economic potential for forage purposes. However, the endophytic and rhizospheric microbial communities within this accession and their ability to promote plant growth remain unknown. The present study aimed to isolate the endophytic and rhizospheric bacteria associated with and to assess their potential for plant growth improvement, so-called plant growth-promoting bacteria (PGPB). For the tests, the ability of nitrogen-fixing bacteria (NFB), phosphate solubilization (PS) and indoleacetic acid (IAA) production were evaluated. A total of 116 endophytic and rhizosphere bacteria were obtained from the isolation. In the tests, 43 (37.00%) of these isolates showed positive NFB, PS, and IAA results. These isolates were identified by 16S rDNA sequencing. The phosphate solubilization index (PSI) ranged from 2 to 3.61, all 43 strains performed biological nitrogen fixation and the IAA production ranged from 12.85 to 431.41 μg ml. Eight of these 43 isolates were evaluated in a greenhouse using caryopsis. The pots were filled with soil prepared with three different phosphate sources and one control without phosphate. After growth, the plants were submitted to morphological, bromatological and chemical determination. Data were analyzed using analysis of variance (ANOVA) and principal component analysis (PCA). In the test, treatments 105 ( sp.) and 458 ( sp.) were the most significant for the crystalline phosphate source, 109 ( sp.) for the sedimentary phosphate source and, as for the soluble phosphate source most treatments that received bacterial isolates had higher phosphorus content in the dry matter than the uninoculated soluble phosphate control. The 105FCR (crystalline phosphate + sp.), 109FSE (sedimentary phosphate + sp.), and 110 FSE (sedimentary phosphate + sp.) treatments showed the best results for plant growth promotion. This work made it possible to determine the bacterial community associated with (BGP308) and to obtain new potential plant growth-promoting strains.
该属属于禾本科，有几个原产于巴西的物种。巴西农业研究公司的种质库（GB）包含来自50个物种的约450份种质。在这些种质中，（BGP 308）具有作为饲料的经济潜力。然而，该种质内的内生和根际微生物群落及其促进植物生长的能力尚不清楚。本研究旨在分离与相关的内生和根际细菌，并评估它们改善植物生长的潜力，即所谓的植物促生细菌（PGPB）。对于测试，评估了固氮细菌（NFB）的能力、磷溶解（PS）和吲哚乙酸（IAA）的产生。从分离中总共获得了116株内生和根际细菌。在测试中，这些分离物中有43株（37.00%）的NFB、PS和IAA结果呈阳性。通过16S rDNA测序对这些分离物进行了鉴定。磷溶解指数（PSI）范围为2至3.61，所有43株菌株都进行生物固氮，IAA产量范围为12.85至431.41μg/ml。在温室中使用颖果对这43株分离物中的8株进行了评估。花盆中填充了用三种不同磷源制备且一种不含磷的对照的土壤。生长后，对植物进行形态学、营养学和化学测定。使用方差分析（ANOVA）和主成分分析（PCA）对数据进行分析。在测试中，对于结晶磷源，处理105（种）和458（种）最为显著，对于沉积磷源，处理109（种）最为显著，对于可溶性磷源，大多数接受细菌分离物处理的干物质中磷含量高于未接种的可溶性磷对照。处理105FCR（结晶磷 + 种）、109FSE（沉积磷 + 种）和110 FSE（沉积磷 + 种）在促进植物生长方面显示出最佳结果。这项工作使得确定与（BGP308）相关的细菌群落并获得新的潜在植物促生菌株成为可能。
