Patil Aishwarya, Satish B N V S, Kondody Rony Tomy, Wali Vishal
Department of Oral Pathology and Microbiology, HKES's S Nijalingappa Dental College, Bengaluru, Karnataka, India.
Department of Orthodontics and Dentofacial Orthopaedics, Sri Rajiv Gandhi College of Dental Sciences and Hospital, Bengaluru, India.
J Oral Maxillofac Pathol. 2022 Apr-Jun;26(2):192-198. doi: 10.4103/jomfp.jomfp_48_21. Epub 2022 Jun 28.
Estimation of age is an important aspect in determining a person's identity. Since teeth are resistant to decay and degradation, estimation of age using dental structures may be a valuable tool in human identification. Recent research suggests that tooth cemental annulation could be used as an effective method for age estimation than other morphologic or histological parameters.
A total of 100 extracted teeth were chosen. Using a hard tissue microtome (SP 1600) longitudinal and transverse ground sections of 100 μm thickness of each tooth were prepared. It is further examined under light microscopy, polarized microscopy and phase-contrast microscopy. The images of longitudinal and transverse were magnified on a computer and the cemental lines were counted.
The present study showed is a strong positive correlation for cemental annulation between the calculated age and estimated age when phase-contrast microscopy was used in both longitudinal and transverse sections when compared with other microscopes. The correlation coefficient value was found more significant in the longitudinal section which implies that the phase-contrast microscope was highly reliable and had a significant correlation ( < 0.001) with the actual age compared to other microscopes.
Cemental annulation by various microscopes improves accuracy in the prediction of age and also it allows for age estimation in cases where skeletal fragments are poorly preserved.
年龄估计是确定个人身份的一个重要方面。由于牙齿抗腐烂和降解，利用牙齿结构进行年龄估计可能是人类身份识别中的一种有价值的工具。最近的研究表明，与其他形态学或组织学参数相比，牙齿牙骨质年轮可作为一种有效的年龄估计方法。
共选取100颗拔除的牙齿。使用硬组织切片机（SP 1600）为每颗牙齿制备厚度为100μm的纵向和横向磨片。进一步在光学显微镜、偏光显微镜和相差显微镜下进行检查。将纵向和横向的图像在计算机上放大并计算牙骨质线。
本研究表明，与其他显微镜相比，在纵向和横向切片中使用相差显微镜时，计算年龄与估计年龄之间的牙骨质年轮呈强正相关。纵向切片中的相关系数值更显著，这意味着相差显微镜高度可靠，与实际年龄的相关性显著（<0.001）。
通过各种显微镜观察牙骨质年轮可提高年龄预测的准确性，并且在骨骼碎片保存不佳的情况下也可进行年龄估计。
