长链非编码 RNA AFAP1-AS1 在喉癌中的表达影响 TU212 细胞系的增殖、侵袭、迁移和凋亡。

The Expression of the Long Noncoding RNA AFAP1-AS1 in Laryngeal Carcinoma Affects the Proliferation, Invasion, Migration, and Apoptosis of TU212 Cell Line.

机构信息

Otolaryngology Department of Youxian People's Hospital of Hunan Province, China.

Department of Otolaryngology and Head and Neck Surgery, Guangzhou Red Cross Hospital Affiliated to Jinan University, China.

出版信息

Biomed Res Int. 2022 Aug 4;2022:2337447. doi: 10.1155/2022/2337447. eCollection 2022.

BACKGROUND

lncRNA AFAP1-AS1 has been linked to the pathogenesis of a wide range of tumors. Nevertheless, whether it plays a role in laryngeal carcinoma (LC) remains unclear.

METHODS

Twenty-nine pairs of LC and related normal tissues were collected for the detection of lncRNA AFAP1-AS1 using qRT-PCR. Correlation of lncRNA AFAP1-AS1 level and clinicopathological characters was assessed by the chi-square test. Impacts of lncRNA AFAP1-AS1 silencing on LC phenotypes were tested via CCK-8, clone formation, EdU staining, wound healing, flow cytometry, and Transwell assay.

RESULTS

Herein, a remarkable elevation of lncRNA AFAP1-AS1 was observed in LC patients. And higher lncRNA AFAP1-AS1 level was correlated to worse clinical pathological characteristics. Moreover, lncRNA AFAP1-AS1 silencing was revealed to repress TU212 malignant phenotypes.

CONCLUSION

Our data suggested that lncRNA AFAP1-AS1 acts as an oncogene of LC .

背景

长链非编码 RNA AFAP1-AS1 与多种肿瘤的发病机制有关。然而，它在喉癌（LC）中的作用尚不清楚。

方法

收集 29 对 LC 及相关正常组织，采用 qRT-PCR 检测 lncRNA AFAP1-AS1。采用卡方检验评估 lncRNA AFAP1-AS1 水平与临床病理特征的相关性。通过 CCK-8、克隆形成、EdU 染色、划痕愈合、流式细胞术和 Transwell 检测，检测 lncRNA AFAP1-AS1 沉默对 LC 表型的影响。