Physics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, 4710-057 Braga, Portugal.
LaPMET - Laboratory of Physics for Materials and Emergent Technologies, University of Minho, 4710-057 Braga, Portugal.
J Mater Chem B. 2022 Aug 31;10(34):6472-6482. doi: 10.1039/d2tb01155g.
Cardiac tissue regeneration strategies are increasingly taking advantage of electroactive scaffolds to actively recreate the tissue microenvironment. In this context, this work reports on advanced materials based on two different ionic liquids (ILs), 2-hydroxyethyl-trimethylammonium dihydrogen phosphate ([Ch][DHP]) and choline bis(trifluoromethylsulfonyl)imide ([Ch][TFSI]), combined with poly(vinylidene fluoride--trifluoroethylene) (P(VDF-TrFE)) for the development of ionic electroactive IL/polymer hybrid materials for cardiac tissue engineering (TE). The morphological, physico-chemical, thermal and electrical properties of the hybrid materials, as well as their potential use as scaffolds for cardiac TE applications, were evaluated. Besides inducing changes in surface topography, roughness and wettability of the composites, the incorporation of [Ch][DHP] and [Ch][TFSI] leads to the increase in surface () and volume () electrical conductivities. Furthermore, washing the hybrid samples with phosphate-buffered saline solution strongly decreases the , whereas and of the composites remain almost unaltered after exposure to ultraviolet sterilization treatment. Additionally, it is verified that the incorporation of IL influences the P(VDF-TrFE) microstructure and crystallization process, acting as a defect during its crystallization. Cytotoxicity assays revealed that hybrid films based on [Ch][DHP] alone are not cytotoxic. These films also support H9c2 myoblast cell adhesion and proliferation, demonstrating their suitability for cardiac TE strategies based on electroactive microenvironments.
心脏组织再生策略越来越多地利用电活性支架来主动重建组织微环境。在这种情况下，本工作报道了基于两种不同离子液体（ILs）的先进材料，即 2-羟乙基三甲基氯化铵二氢磷酸盐（[Ch][DHP]）和胆碱双（三氟甲烷磺酰基）亚胺（[Ch][TFSI]），与聚偏二氟乙烯-三氟乙烯（P（VDF-TrFE））相结合，用于开发用于心脏组织工程（TE）的离子电活性 IL/聚合物杂化材料。评估了杂化材料的形态、物理化学、热学和电学性质，以及它们作为心脏 TE 应用支架的潜在用途。除了诱导复合材料表面形貌、粗糙度和润湿性的变化外，[Ch][DHP]和[Ch][TFSI]的掺入还导致表面（）和体积（）电导率的增加。此外，用磷酸盐缓冲盐水（PBS）洗涤杂化样品会强烈降低 ，而暴露于紫外线灭菌处理后，复合材料的 和 几乎保持不变。此外，验证了 IL 的掺入会影响 P（VDF-TrFE）的微观结构和结晶过程，在其结晶过程中充当缺陷。细胞毒性试验表明，仅基于[Ch][DHP]的杂化膜没有细胞毒性。这些薄膜还支持 H9c2 成肌细胞的黏附和增殖，证明它们适合基于电活性微环境的心脏 TE 策略。
