NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases (First Affiliated Hospital, School of Medicine, Shihezi University); Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, People's Republic of China.
Department of Neurology, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, People's Republic of China.
Physiol Genomics. 2022 Oct 1;54(10):371-379. doi: 10.1152/physiolgenomics.00089.2021. Epub 2022 Aug 15.
As a major complication after percutaneous coronary intervention (PCI) in patients who suffer from coronary artery disease, in-stent restenosis (ISR) poses a significant challenge for clinical management. A miRNA-mRNA regulatory network of ISR can be constructed to better reveal the occurrence of ISR. The relevant data set from the Gene Expression Omnibus (GEO) database was downloaded, and 284 differentially expressed miRNAs (DE-miRNAs) and 849 differentially expressed mRNAs (DE-mRNAs) were identified. As predicted by online tools, 65 final functional genes (FmRNAs) were overlapping DE-mRNAs and DE-miRNAs target genes. In the biological process (BP) terms of gene ontology (GO) functional analysis, the FmRNAs were mainly enriched in the cellular response to peptide, epithelial cell proliferation, and response to peptide hormone. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the FmRNAs were mainly enriched in breast cancer, endocrine resistance, and Cushing syndrome. Jun proto-oncogene, activator protein-1 (AP-1) transcription factor subunit (), insulin-like growth factor 1 receptor (), member oncogene family (), specificity protein 1 (), protein tyrosine phosphatase nonreceptor type 1 (), DDB1 and CUL4 associated factor 10 (), retinoblastoma-binding protein 5 (), and eukaryotic initiation factor 4A-I () were hub genes in the protein-protein interaction network (PPI network). The miRNA-mRNA network containing DE-miRNAs and hub genes was built. Hsa-miR-139-5p-, hsa-miR-324-5p- axis pairs were found in the miRNA-mRNA network, which could promote ISR development. The aforementioned results indicate that the miRNA-mRNA network constructed in ISR has a regulatory role in the development of ISR and may provide new approaches for clinical treatment and experimental development.
作为冠心病患者经皮冠状动脉介入治疗（PCI）后的主要并发症，支架内再狭窄（ISR）对临床管理构成了重大挑战。可以构建 ISR 的 miRNA-mRNA 调控网络，以更好地揭示 ISR 的发生。从基因表达综合数据库（GEO）数据库中下载了相关数据集，鉴定出 284 个差异表达 miRNA（DE-miRNA）和 849 个差异表达 mRNA（DE-mRNA）。通过在线工具预测，65 个最终功能基因（FmRNAs）重叠了 DE-mRNAs 和 DE-miRNA 靶基因。在基因本体论（GO）功能分析的生物学过程（BP）术语中，FmRNAs 主要富集于细胞对肽的反应、上皮细胞增殖和对肽激素的反应。在京都基因与基因组百科全书（KEGG）分析中，FmRNAs 主要富集于乳腺癌、内分泌抵抗和库欣综合征。原癌基因 Jun、激活蛋白-1（AP-1）转录因子亚基（）、胰岛素样生长因子 1 受体（）、成员 原癌基因家族（）、特异性蛋白 1（）、蛋白酪氨酸磷酸酶非受体型 1（）、DDB1 和 CUL4 相关因子 10（）、视网膜母细胞瘤结合蛋白 5（）和真核起始因子 4A-I（）是蛋白质-蛋白质相互作用网络（PPI 网络）中的枢纽基因。构建了包含 DE-miRNA 和枢纽基因的 miRNA-mRNA 网络。在 miRNA-mRNA 网络中发现了 hsa-miR-139-5p-、hsa-miR-324-5p- 轴对，可促进 ISR 发展。上述结果表明，ISR 中构建的 miRNA-mRNA 网络在 ISR 发展中具有调节作用，可为临床治疗和实验开发提供新方法。
