Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
Institute of Cellular and Molecular Biology, Collage of Life Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China.
J Agric Food Chem. 2022 Aug 24;70(33):10248-10258. doi: 10.1021/acs.jafc.2c02077. Epub 2022 Aug 15.
Amino acid sensing plays an important role in regulating lipid metabolism by sensing amino acid nutrient disturbance. T1R1 (umami taste receptor, type 1, member 1) is a membrane G protein-coupled receptor that senses amino acids. -knockout (KO) mice were used to explore the function of umami receptors in lipid metabolism. Compared with wild-type (WT) mice, -KO mice showed decreased fat mass ( < 0.05) and adipocyte size, lower liver triglyceride (7.835 ± 0.809 12.463 ± 0.916 mg/g WT, = 0.013) and total cholesterol levels (0.542 ± 0.109 1.472 ± 0.044 mmol/g WT, < 0.001), and reduced lipogenesis gene expressions in adipose and liver tissues. Targeted liver amino acid metabolomics showed that the amino acid content of -KO mice was significantly decreased, which was consistent with the branched-chain ketoacid dehydrogenase protein levels. Proteomics analysis showed that the upregulated proteins were enriched in lipid and steroid metabolism pathways, and parallel reaction monitoring results illustrated that ablation promoted lipid catabolism through oxysterol 7 α-hydroxylase and insulin-like growth factor binding protein 2. In summary, disruption in mice could reduce lipid accumulation by reducing lipid synthesis and improving lipid catabolism.
氨基酸感应在调节脂质代谢中起着重要作用，通过感应氨基酸营养失调。T1R1（鲜味味觉受体，类型 1，成员 1）是一种膜 G 蛋白偶联受体，可感应氨基酸。使用 -knockout（KO）小鼠来探索鲜味受体在脂质代谢中的功能。与野生型（WT）小鼠相比，-KO 小鼠的脂肪质量减少（ < 0.05），脂肪细胞大小减小，肝甘油三酯（7.835 ± 0.809 12.463 ± 0.916 mg/g WT， = 0.013）和总胆固醇水平降低（0.542 ± 0.109 1.472 ± 0.044 mmol/g WT， < 0.001），脂肪和肝脏组织中的脂肪生成基因表达减少。靶向肝脏氨基酸代谢组学显示 -KO 小鼠的氨基酸含量明显降低，与支链酮酸脱氢酶蛋白水平一致。蛋白质组学分析表明，上调的蛋白质富集在脂质和类固醇代谢途径中，平行反应监测结果表明，缺失通过氧化固醇 7α-羟化酶和胰岛素样生长因子结合蛋白 2 促进脂质分解代谢。总之，小鼠中的缺失可以通过减少脂质合成和改善脂质分解代谢来减少脂质积累。
