van Overveld F J, Bruijnzeel P L, Kreukniet J, Raaijmakers J A, Terpstra G K
Int J Tissue React. 1987;9(1):61-7.
An isolation procedure to obtain inflammatory cells from normal human lung tissue is described, consisting of four steps: an enzymatic digestion, centrifugal elutriation, gradient centrifugation and additional affinity chromatography (to obtain mast cells). The dispersed cell population consisted mainly of macrophages, pneumocytes type II, neutrophils and lymphocytes. Further separation by elutriation yielded pure macrophages and pure lymphocytes. The heterogeneous cell mixtures obtained at elutriation were separated by gradient centrifugation. This method yielded pure macrophages, lymphocytes and pneumocytes, and fractions containing eosinophils, mast cells or neutrophils were enriched considerably. Additional affinity chromatography with anti-human-IgE yielded a mast-cell fraction of 75%.
本文描述了一种从正常人肺组织中获取炎性细胞的分离程序，该程序包括四个步骤：酶消化、离心淘析、梯度离心和额外的亲和层析（用于获取肥大细胞）。分散的细胞群体主要由巨噬细胞、II型肺细胞、中性粒细胞和淋巴细胞组成。通过淘析进一步分离可得到纯巨噬细胞和纯淋巴细胞。在淘析过程中获得的异质细胞混合物通过梯度离心进行分离。该方法可产生纯巨噬细胞、淋巴细胞和肺细胞，并且含有嗜酸性粒细胞、肥大细胞或中性粒细胞的组分得到了显著富集。用抗人IgE进行的额外亲和层析产生了75%的肥大细胞组分。
