Undem B J, Green F, Warner T, Buckner C K, Graziano F M
J Immunol Methods. 1985 Aug 2;81(2):187-97. doi: 10.1016/0022-1759(85)90203-0.
Pulmonary mast cells were obtained from guinea pig lung using a combination of enzymatic digestion of tissue, centrifugal elutriation, and density gradient centrifugation on Percoll. In the initial procedure, lung tissue was enzymatically digested with collagenase and elastase in four 30 min incubations. Typically, monodispersed cell suspensions contained 4% mast cells. Further purification of these lung mast cells using elutriation and Percoll gradients consistently yielded mast cells of 40-78% (mean 51%) purity. These cells were morphologically intact, viable and found to be functional as determined by histamine release evoked by antigen and anti-guinea pig IgG1 antibody.
通过组织酶消化、离心淘析以及在Percoll上进行密度梯度离心相结合的方法，从豚鼠肺中获取肺肥大细胞。在初始步骤中，肺组织用胶原酶和弹性蛋白酶进行四次30分钟的酶消化。通常，单分散细胞悬液中含有4%的肥大细胞。使用淘析和Percoll梯度对这些肺肥大细胞进一步纯化，始终能得到纯度为40 - 78%（平均51%）的肥大细胞。这些细胞形态完整、有活力，并且通过抗原和抗豚鼠IgG1抗体诱发的组胺释放测定发现其具有功能。
