Departamento de Química, Instituto Universitario de Oceanografía y Cambio Global, Universidad de Las Palmas de Gran Canaria, Campus de Tafira, Las Palmas de Gran Canaria, Canary Islands, Spain.
Chem Biodivers. 2022 Sep;19(9):e202200230. doi: 10.1002/cbdv.202200230. Epub 2022 Aug 31.
Three freshwater microalgae (Spirogyra sp., Cosmarium sp., and Cosmarium blytii) collected from several locations in Gran Canaria have been studied to explore their potential as a novel source of bioactive compounds for biotechnological applications. Soluble carbohydrates were quantified after extraction with 3 M HCl at 100 °C, ranging from 35.8 to 43.3 %, and with water at room temperature, ranging from 19 to 22.8 %. Amino acids glutamic acid, proline and aspartic acid were quantified by RP-HPLC. Glutamic acid was the most abundant, ranging from 12.2 to 3.63 mg g of dry biomass. Cosmarium blytii was the richest sample in amino acids (24.02 mg g of dry weight). In addition, Cosmarium blytii and Spyrogira sp. exhibited higher radical scavenging activity (RSA) against 1,1-diphenyl-2-picrylhydrazyl (DPPH) than that of the synthetic antioxidant butylhydroxytoluene (BHT), commonly used as food additive. These results show a great potential of these microalgae for exploitation in the food, feed and pharmaceutical industries.
从大加那利岛的几个地点采集的三种淡水微藻（水绵属、角星鼓藻和比氏角星鼓藻）被研究探索它们作为生物技术应用中生物活性化合物的新型来源的潜力。在 100°C 下用 3M HCl 提取后，可定量测定可溶性碳水化合物，范围为 35.8 至 43.3%，在室温下用热水提取，范围为 19 至 22.8%。通过反相高效液相色谱法（RP-HPLC）定量测定氨基酸谷氨酸、脯氨酸和天冬氨酸。谷氨酸含量最丰富，范围为 12.2 至 3.63mg/g 干生物质。比氏角星鼓藻是氨基酸含量最丰富的样品（24.02mg/g 干重）。此外，比氏角星鼓藻和水绵属对 1,1-二苯基-2-苦基肼基（DPPH）的自由基清除活性（RSA）高于常用作食品添加剂的合成抗氧化剂丁基羟基甲苯（BHT）。这些结果表明这些微藻在食品、饲料和制药工业中有很大的开发潜力。
