Goetsch A L, Jones A L, Beers K W, Stokes S R, Piper E L
J Anim Sci. 1987 Jun;64(6):1769-78. doi: 10.2527/jas1987.6461769x.
Ten Holstein steers (141 kg) were used in two 5 X 5 Latin-square experiments conducted simultaneously to determine the effects of offering different levels and types of feeds with endophyte-infected fescue given ad libitum. In Exp. 1, steers were given ad libitum access to infected fescue hay in the afternoon; in the morning fescue was given ad libitum (basal) or bermudagrass or clover hays were fed at .5 or 1.0% of body weight (BW). Supplementation did not affect total dry matter intake (P greater than .10), but supplementation at 1.0% of BW yielded total intake greater than supplementation at .5% of BW (P less than .05). Supplementation did not change digestibilities of dry or organic matter (P greater than .10). Particulate passage rate was greater (P less than .10) with supplementation at 1.0 than at .5% of BW, and increasing the level of supplementation from .5 to 1.0% of BW affected fluid passage rate positively with clover but negatively with bermudagrass (interaction, P less than .05). Serum prolactin increased (P less than .05) with all supplementation treatments, although no differences were observed between supplement type-supplementation level combinations (P greater than .10). Ground corn and wheat hay were supplements in Exp. 2. Total intake of dry matter was greater with supplements provided at 1.0 rather than at .5% of BW and for corn rather than wheat hay (P less than .05). Neutral detergent fiber digestion (percent of intake and grams per day) rose when wheat hay was offered at 1.0 vs .5% of BW but declined when the level of supplemental corn increased from .5 to 1.0% of BW (interaction, P less than .05). There were no differences among diets in particulate and fluid passage rates and serum prolactin concentration. Supplementation with nontoxic forage of a basal diet of infected fescue yielded intake substitution when forage was offered at .5% of BW, although incomplete substitution occurred with 1.0% of BW of supplemental forage such that total intake increased as compared to the lower level of supplementation.
选用10头荷斯坦阉牛（体重141千克），同时进行了两项5×5拉丁方试验，以确定随意提供不同水平和类型的饲料以及内生真菌感染的羊茅对其的影响。在试验1中，下午让阉牛随意采食感染羊茅的干草；上午随意提供羊茅（基础日粮），或以体重（BW）的0.5%或1.0%投喂百慕大草或三叶草干草。补充饲料对总干物质摄入量没有影响（P>0.10），但以体重的1.0%补充饲料时的总摄入量高于以体重的0.5%补充饲料时的总摄入量（P<0.05）。补充饲料对干物质或有机物的消化率没有影响（P>0.10）。以体重的1.0%补充饲料时的颗粒通过率高于以体重的0.5%补充饲料时的颗粒通过率（P<0.10），并且将补充饲料水平从体重的0.5%提高到1.0%，对三叶草的液体通过率有积极影响，而对百慕大草的液体通过率有消极影响（交互作用，P<0.05）。所有补充饲料处理组的血清催乳素均升高（P<0.05），尽管在补充饲料类型-补充饲料水平组合之间未观察到差异（P>0.10）。在试验2中，补充饲料为玉米粉和小麦干草。以体重的1.0%而不是0.5%提供补充饲料时，干物质的总摄入量更高，并且投喂玉米时的总摄入量高于投喂小麦干草时的总摄入量（P<0.05）。当以体重的1.0%而不是0.5%投喂小麦干草时，中性洗涤纤维消化率（摄入量的百分比和每天的克数）升高，但当补充玉米的水平从体重的0.5%增加到1.0%时，中性洗涤纤维消化率下降（交互作用，P<0.05）。不同日粮之间在颗粒和液体通过率以及血清催乳素浓度方面没有差异。当以体重的0.5%投喂补充饲料时，用无毒饲料补充感染羊茅的基础日粮会产生摄入量替代，尽管当补充饲料为体重的1.0%时发生不完全替代，以至于与较低水平的补充饲料相比，总摄入量增加。
