College of Environment, Zhejiang University of Technology, Hangzhou310014, China.
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education; College of Chemistry, Central China Normal University, Wuhan430079, China.
J Agric Food Chem. 2022 Aug 24;70(33):10134-10143. doi: 10.1021/acs.jafc.2c02489. Epub 2022 Aug 16.
Harmful cyanobacterial blooms (HCBs) caused by are of great concern as they negatively affect the aquatic environment and human health. Chemical methods could rapidly eradicate HCBs and have been used for many decades. However, many chemical reagents are not recommended to eliminate HCBs in the long term, given the possible destructive and toxic effects of the chemicals employed on non-target aquatic organisms. We developed a new algaecide, 2-((1,3,4-thiadiazol-2-yl)thio)--(4-chlorophenyl) acetamide (Q2), to control harmful cyanobacteria while being environmentally friendly and selective. In our study, Q2 effectively inhibited cyanobacterial growth, especially of , but did not affect eukaryotic algae in test concentrations. A critical mechanism was revealed by transcriptome and metagenomic results showing that Q2 affects multiple cellular targets of cyanobacteria for HCB control, including the destruction of organelles, damage in the photosynthesis center, as well as inhibition of gas vesicle growth, and these changes can be highly relevant to the decrease of quorum-sensing functional KEGG pathways. Furthermore, Q2 did not affect the microbial composition and could recover the disrupted aquatic functional pathways in a short period. This is different from the impact on ecosystem functioning of the traditionally used harmful algaecide diuron. All these results verified that Q2 could be friendly to the aquatic environment, providing a new directional choice in managing HCBs in the future.
由 引起的有害蓝藻水华（HCBs）令人担忧，因为它们会对水生环境和人类健康产生负面影响。化学方法可以快速消除 HCBs，并且已经使用了几十年。然而，鉴于所使用的化学物质对非目标水生生物可能具有破坏性和毒性作用，许多化学试剂不建议长期用于消除 HCBs。我们开发了一种新的杀藻剂 2-((1,3,4-噻二唑-2-基)硫基)-(4-氯苯基)乙酰胺（Q2），以在环保和选择性的同时控制有害蓝藻。在我们的研究中，Q2 有效地抑制了蓝藻的生长，特别是 ，但在测试浓度下不会影响真核藻类。转录组和宏基因组结果揭示了一个关键机制，表明 Q2 影响蓝藻控制 HCB 的多个细胞靶标，包括细胞器的破坏、光合作用中心的损伤以及气室生长的抑制，这些变化与群体感应功能 KEGG 途径的减少高度相关。此外，Q2 不会影响微生物组成，并可以在短时间内恢复被破坏的水生功能途径。这与传统使用的有害杀藻剂敌草隆对生态系统功能的影响不同。所有这些结果都证实了 Q2 可以对水生环境友好，为未来管理 HCBs 提供了新的方向选择。
