Department of Anatomy, University of Otago, Dunedin 9016, New Zealand.
Department of Zoology, University of Otago, Dunedin 9016, New Zealand.
G3 (Bethesda). 2022 Sep 30;12(10). doi: 10.1093/g3journal/jkac199.
The European earwig Forficula auricularia is an important model for studies of maternal care, sexual selection, sociality, and host-parasite interactions. However, detailed genetic investigations of this species are hindered by a lack of genomic resources. Here, we present a high-quality hybrid genome assembly for Forficula auricularia using Nanopore long-reads and 10× linked-reads. The final assembly is 1.06 Gb in length with 31.03% GC content. It consists of 919 scaffolds with an N50 of 12.55 Mb. Half of the genome is present in only 20 scaffolds. Benchmarking Universal Single-Copy Orthologs scores are ∼90% from 3 sets of single-copy orthologs (eukaryotic, insect, and arthropod). The total repeat elements in the genome are 64.62%. The MAKER2 pipeline annotated 12,876 protein-coding genes and 21,031 mRNAs. Phylogenetic analysis revealed the assembled genome as that of species B, one of the 2 known genetic subspecies of Forficula auricularia. The genome assembly, annotation, and associated resources will be of high value to a large and diverse group of researchers working on dermapterans.
欧洲耳夹子蜚蠊（Forficula auricularia）是研究亲代关怀、性选择、社会性以及宿主-寄生虫相互作用的重要模式生物。然而，由于缺乏基因组资源，对该物种的详细遗传研究受到了阻碍。在这里，我们使用纳米孔长读长和 10x 连接读长为欧洲耳夹子蜚蠊（Forficula auricularia）提供了高质量的混合基因组组装。最终组装的基因组大小为 1.06GB，GC 含量为 31.03%。它由 919 个支架组成，N50 为 12.55Mb。基因组的一半仅存在于 20 个支架中。通用单拷贝同源物（universal single-copy orthologs）的基准测试分数在 3 组单拷贝同源物（真核生物、昆虫和节肢动物）中约为 90%。基因组中的总重复元件为 64.62%。MAKER2 流水线注释了 12876 个蛋白质编码基因和 21031 个 mRNA。系统发育分析表明，组装的基因组是 2 个已知的欧洲耳夹子蜚蠊（Forficula auricularia）遗传亚种之一 B 种的基因组。该基因组组装、注释和相关资源将对研究 Dermaptera 目昆虫的广大多样的研究群体具有很高的价值。
