Li Shuman, Liu Bin, Liu Yongjie, Ding Yu-Qiang, Zhang Jun, Feng Liping
Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, School of Public Health, Shanghai Jiao-Tong University School of Medicine, Shanghai, China.
Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai, China.
Environ Geochem Health. 2023 May;45(5):2387-2400. doi: 10.1007/s10653-022-01352-3. Epub 2022 Aug 16.
The association between exposure to particulate matter (PM) during pregnancy and abnormal birth outcomes is still inconclusive. This study aims to provide more evidence for this public health concern by investigating birth outcomes and the growth of offspring in mice exposed to PM during pregnancy. C57BL/6 J pregnant mice were exposed to PM via nasal drip at three doses or solvent control. The dam weight gain was recorded during pregnancy. The number of pups, pup weight, and placental weight were recorded at embryonic day 18.5 (E18.5) necropsy. For mice that gave birth naturally, we calculated the gestation length and measured the body weight of offspring once a week from the 1st to the 6th week after birth. The results showed that there were no significant differences in maternal body weight gain, conception rate, pregnancy duration, and litter size among different groups. There were no significant differences in fetal weight, placental weight, and fetal/placental weight ratio at E18.5. Weight gain in offspring was reduced after birth. The average body weight of offspring in the high-dose group was significantly lower than that in the control group at weeks 5 in female pups. There were no significant differences in the body weight of male offspring among groups from 1st to the 6th. Together, our study indicated that maternal exposure to PM did not significantly impact birth outcomes of C57BL/6 J mice but affected growth trajectories in offspring after birth in a dose- and fetal sex-dependent manner.
孕期暴露于颗粒物（PM）与异常出生结局之间的关联仍无定论。本研究旨在通过调查孕期暴露于PM的小鼠的出生结局和子代生长情况，为这一公共卫生问题提供更多证据。将C57BL/6 J孕鼠通过滴鼻法暴露于三种剂量的PM或溶剂对照。在孕期记录母鼠体重增加情况。在胚胎第18.5天（E18.5）尸检时记录幼崽数量、幼崽体重和胎盘重量。对于自然分娩的小鼠，我们计算妊娠期长度，并在出生后第1周至第6周每周测量一次子代体重。结果显示，不同组之间母鼠体重增加、受孕率、妊娠期和窝仔数均无显著差异。在E18.5时，胎儿体重、胎盘重量和胎儿/胎盘重量比均无显著差异。出生后子代体重增加减少。在雌性幼崽中，高剂量组子代在第5周时的平均体重显著低于对照组。在第1周至第6周期间，雄性子代体重在各组之间无显著差异。总之，我们的研究表明，母鼠暴露于PM对C57BL/6 J小鼠的出生结局没有显著影响，但以剂量和胎儿性别依赖的方式影响出生后子代的生长轨迹。
