Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran.
Neurophysiology Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran.
Cell Stress Chaperones. 2022 Sep;27(5):513-521. doi: 10.1007/s12192-022-01287-9. Epub 2022 Aug 16.
Diabetic peripheral neuropathy (DPN) is a common nerve disorder of diabetes. The aim of this study was to explore the protective effects of tropisetron in DPN. Type 1 diabetes was created by a single injection of streptozotocin (50 mg/kg, ip). Tropisetron (3 mg/kg, ip) was administered daily for 2 weeks. Our analysis showed that nerve fibers and their myelin sheaths were thinned with decreased myelinated fiber number in diabetic animals. The intensity of Bcl-2 staining decreased and the intensity of Bax staining increased in the sciatic nerves of diabetic rats by using immunohistochemical staining. Furthermore, diabetes significantly increased tumor necrosis factor-alpha, interleukin 1-β (TNFα and IL-1β) and Bax/Bcl-2 ratio in sciatic nerves of rats. However, intraperitoneal injection of tropisetron significantly reversed these alterations induced by diabetes. These findings suggest that tropisetron attenuates diabetes-induced peripheral nerve injury through its anti-inflammatory and anti-apoptotic effects, and may provide a novel therapeutic strategy to ameliorate the process of peripheral neuropathy in diabetes.
糖尿病周围神经病变（DPN）是糖尿病常见的神经病变。本研究旨在探讨曲匹司特对 DPN 的保护作用。采用链脲佐菌素（50mg/kg，ip）单次注射建立 1 型糖尿病模型。曲匹司特（3mg/kg，ip）每日给药 2 周。我们的分析表明，糖尿病动物的神经纤维及其髓鞘变薄，有髓神经纤维数量减少。免疫组织化学染色显示，糖尿病大鼠坐骨神经中 Bcl-2 染色强度降低，Bax 染色强度增加。此外，糖尿病大鼠坐骨神经中肿瘤坏死因子-α、白细胞介素 1-β（TNFα 和 IL-1β）和 Bax/Bcl-2 比值显著升高。然而，曲匹司特腹腔注射可显著逆转糖尿病引起的这些改变。这些发现表明，曲匹司特通过抗炎和抗细胞凋亡作用减轻糖尿病引起的周围神经损伤，可能为改善糖尿病周围神经病变过程提供一种新的治疗策略。
