Department of Biological Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Department of Biomedical Informatics, Harvard Medical School, Boston, Massachusetts 02115, United States.
Environ Sci Technol. 2022 Sep 6;56(17):12137-12147. doi: 10.1021/acs.est.2c00821. Epub 2022 Aug 16.
Pesticides are critical for invasive species management but often have negative effects on nontarget native biota. Tolerance to pesticides should have an evolutionary basis, but this is poorly understood. Invasive sea lamprey () populations in North America have been controlled with a pesticide lethal to them at lower concentrations than native fishes. We addressed how interspecific variation in gene expression and detoxification gene diversity confer differential pesticide sensitivity in two fish species. We exposed sea lamprey and bluegill (), a tolerant native species, to 3-trifluoromethyl-4-nitrophenol (TFM), a pesticide commonly used in sea lamprey control. We then used whole-transcriptome sequencing of gill and liver to characterize the cellular response in both species. Comparatively, bluegill exhibited a larger number of detoxification genes expressed and a larger number of responsive transcripts overall, which likely contributes to greater tolerance to TFM. Understanding the genetic and physiological basis for pesticide tolerance is crucial for managing invasive species.
农药对于入侵物种管理至关重要，但通常对非目标本地生物群具有负面影响。对农药的耐受性应该具有进化基础，但这一点了解甚少。北美的入侵海七鳃鳗种群已经通过一种对它们有致命作用的农药得到控制，这种农药对本地鱼类的致死浓度要低得多。我们研究了两个鱼类物种的基因表达和解毒基因多样性的种间差异如何赋予其对农药敏感性的差异。我们将海七鳃鳗和蓝鳃太阳鱼（一种耐受的本地物种）暴露于三氟甲基-4-硝基苯酚（TFM）中，这是一种常用于控制海七鳃鳗的农药。然后，我们使用全转录组测序技术对鳃和肝脏进行测序，以描述这两个物种的细胞反应。相比之下，蓝鳃太阳鱼表达的解毒基因数量更多，总体上响应的转录本数量更多，这可能有助于其对 TFM 有更大的耐受性。了解对农药的耐受性的遗传和生理基础对于管理入侵物种至关重要。
