The neurosecretory staining in the pleural-pedal ganglion of the Japanese abalone (Ezoawabi), Haliotis discus hannai, and its relationship to reproduction; with a description of a newly observed neurohemal organ.

Neurosecretory staining in the pleural-pedal ganglion of the Japanese abalone (ezoawabi), Haliotis discus hannai, was studied during the reproductive cycle. The variation in stain affinity of the cells and amount of neurosecretory material contained in the neurosecretory cells were measured during the study. The pleural-pedal ganglion contained 7 different cell types. The quantity of different cell types in the pleural-pedal ganglion was unusual for a prosobranch gastropod. Cell Types, α- and β-cells, showed neurosecretory staining and the stain intensity varied during the reproductive cycle. α-cells were the most abundant cells in the pleural-pedal ganglion and the quantity of neurosecretory material inside the cell body clearly correlated with gametogenesis. Neurosecretory material in β-cells showed a strong correlation with the induction of spawning. A neurohemal organ (pleural organ) was found in close proximity to the β-cells in the statocyst tissue. The pleural organ is the first ectodermal neurohemal organ reported in a prosobranch gastropod. A nervous connection (stato-pleural nerve) with a possible sensory function was found between the statocyst and the pleural-pedal ganglion.