Chambers T J, Fuller K, Darby J A
J Cell Physiol. 1987 Jul;132(1):90-6. doi: 10.1002/jcp.1041320112.
Osteoclasts disaggregated from neonatal rat long bones and incubated on plastic or glass substrates were found to release a considerable proportion of tartrate-resistant acid phosphatase into culture supernatants. Enzyme release was detectable in the supernatant medium of cultures containing as few as ten cells after 1 hr of incubation and proceeded in a linear manner for the ensuing 6 hr. Calcitonin (1 pg/ml) and cytochalasin B (5 micrograms/ml) inhibited release into the supernatant, suggesting that release represents enzyme secretion. Prostaglandin E1 induced transient inhibition followed by recovery; parathyroid hormone and 1,25(OH)2 vitamin D3 were without influence. Acid phosphatase release in these cultures shows a pattern of hormone responsiveness that coincides with the effects of these hormones on bone resorption by isolated osteoclasts. The extent of acid phosphatase release and its regulation by calciotropic hormones imply a central role for acid hydrolase secretion in osteoclastic bone resorption. The experimental system described in this study may facilitate analysis of the pharmacological hormonal and cellular regulation of osteoclastic function.
从新生大鼠长骨中分离出来并在塑料或玻璃基质上培养的破骨细胞，被发现会将相当一部分抗酒石酸酸性磷酸酶释放到培养上清液中。孵育1小时后，在含有低至10个细胞的培养物的上清液培养基中即可检测到酶的释放，并且在接下来的6小时内呈线性释放。降钙素（1皮克/毫升）和细胞松弛素B（5微克/毫升）抑制了酶释放到上清液中，这表明这种释放代表酶的分泌。前列腺素E1诱导短暂抑制后恢复；甲状旁腺激素和1,25(OH)2维生素D3则无影响。这些培养物中酸性磷酸酶的释放显示出一种激素反应模式，这与这些激素对分离的破骨细胞骨吸收的影响相一致。酸性磷酸酶释放的程度及其受钙调节激素的调控意味着酸性水解酶分泌在破骨细胞骨吸收中起核心作用。本研究中描述的实验系统可能有助于分析破骨细胞功能的药理学、激素和细胞调控。
