基于柱前技术和自动柱套更换的用于液相色谱的全自动样品处理系统。

Fully automated sample handling system for liquid chromatography based on pre-column technology and automated cartridge exchange.

作者信息

Nielen M W, Valk A J, Frei R W, Brinkman U A, Mussche P, De Nijs R, Ooms B, Smink W

出版信息

J Chromatogr. 1987 Apr 22;393(1):69-83. doi: 10.1016/s0021-9673(01)94205-0.

Abstract

The design of an automated cartridge exchange module for on-line sample handling in liquid chromatography is described. When combined with a low-cost purge pump, a solvent selection valve and an auto-sampler, a fully automated sample handling system is obtained. Samples are sorbed on a disposable cartridge packed with 40 micron octyl-bonded silica, purged for clean-up and eluted on-line to the analytical column. Unattended operation of the system is demonstrated for various examples, i.e., the determination of anti-epileptic drugs in serum, an anti-cancer drug in plasma, barbiturates in urine, phenylurea herbicides in river water and caffeine in a soft drink.

摘要

本文描述了一种用于液相色谱在线样品处理的自动柱更换模块的设计。当与低成本的净化泵、溶剂选择阀和自动进样器结合使用时，可获得一个全自动的样品处理系统。样品吸附在装有40微米辛基键合硅胶的一次性柱上，进行净化处理后在线洗脱至分析柱。该系统针对各种实例展示了无人值守操作，例如血清中抗癫痫药物的测定、血浆中抗癌药物的测定、尿液中巴比妥类药物的测定、河水中苯脲类除草剂的测定以及软饮料中咖啡因的测定。