Marx A M, Kronberg H, Neuhoff V
J Chromatogr. 1987 May 1;393(3):407-17. doi: 10.1016/s0021-9673(01)94238-4.
A new method for the analysis of the specific activity of amino acids is described. The analysis is carried out by thin-layer chromatography of the dansylated amino acids, computerized fluorescence evaluation and activity measurement by quantitative autoradiography. Quantitative evaluation of the autoradiographs is achieved by careful calibration of the X-ray film blackening. As shown for 14C-labelled phenylalanine and tyrosine, the method allows the simultaneous determination of the specific activity of 22 amino acids. About 10(-13) mol of an amino acid with a specific activity of less than 5 GBq/mmol can be detected and measured by this method.
本文描述了一种分析氨基酸比活度的新方法。该分析通过丹磺酰化氨基酸的薄层色谱法、计算机荧光评估以及定量放射自显影法进行活性测量。通过对X射线胶片黑化进行仔细校准，实现对放射自显影片的定量评估。如对14C标记的苯丙氨酸和酪氨酸所示，该方法可同时测定22种氨基酸的比活度。用此方法可检测和测量比活度小于5GBq/mmol的约10(-13)mol氨基酸。
