通过薄层色谱法和定量放射自显影相结合的方法测定氨基酸的比放射性。

Determination of the specific radioactivity of amino acids by a combination of thin-layer chromatography and quantitative autoradiography.

作者信息

Marx A M, Kronberg H, Neuhoff V

出版信息

J Chromatogr. 1987 May 1;393(3):407-17. doi: 10.1016/s0021-9673(01)94238-4.

Abstract

A new method for the analysis of the specific activity of amino acids is described. The analysis is carried out by thin-layer chromatography of the dansylated amino acids, computerized fluorescence evaluation and activity measurement by quantitative autoradiography. Quantitative evaluation of the autoradiographs is achieved by careful calibration of the X-ray film blackening. As shown for 14C-labelled phenylalanine and tyrosine, the method allows the simultaneous determination of the specific activity of 22 amino acids. About 10(-13) mol of an amino acid with a specific activity of less than 5 GBq/mmol can be detected and measured by this method.

摘要

本文描述了一种分析氨基酸比活度的新方法。该分析通过丹磺酰化氨基酸的薄层色谱法、计算机荧光评估以及定量放射自显影法进行活性测量。通过对X射线胶片黑化进行仔细校准，实现对放射自显影片的定量评估。如对14C标记的苯丙氨酸和酪氨酸所示，该方法可同时测定22种氨基酸的比活度。用此方法可检测和测量比活度小于5GBq/mmol的约10(-13)mol氨基酸。