Department of Medical Biochemistry, School of Medicine, Pamukkale University, Denizli, Turkey.
Department of Internal Medicine, Division of Endocrinology and Metabolism, School of Medicine, Pamukkale University, Denizli, Turkey.
Diagnosis (Berl). 2022 Aug 18;9(4):499-507. doi: 10.1515/dx-2022-0036. eCollection 2022 Nov 1.
Microribonucleic acids (microRNA/miRNA/miR-) are predicted to be useful in the early diagnosis, monitoring, and treatment of diabetic nephropathy (DN). We aimed to investigate the relationship of DN to miR-21-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-126-3p, miR-129-1-3p, miR-137, miR-192-5p, miR-212-3p, and miR-320c.
There were 50 healthy controls and 100 patients with type 2 diabetes mellitus (T2DM). The diabetic patients were divided into three subgroups: normal to mildly increased (A1, n=51), moderately increased (A2, n=25), and severely increased (A3, n=24) albuminuria. The biochemical measurements were analysed using Roche Cobas 8000. The plasma miRNAs were analysed using RT-qPCR based on SYBR green chemistry.
The relative expression of miR-21-3p was significantly lower in the (A3 p=0.005, 6.6-fold decrease) and DN (A1 + A3) (p=0.005, 6.6-fold decrease) groups compared to the controls. The relative expression of miR-192-5p was also significantly lower in the DN group (p=0.027, 2.4-fold decrease) compared to the controls. The area under curve value was 0.726 for miR-21-3p and 0.717 for miR-192-5p for distinguishing the DN group from the controls.
The decreased expressions of miR-21-3p and miR-192-5p are associated with the development of DN and may be potential biomarkers for the early diagnosis of DN.
微小核糖核酸（miRNA/miR-）被预测在糖尿病肾病（DN）的早期诊断、监测和治疗中具有重要作用。本研究旨在探讨 miR-21-3p、miR-29a-3p、miR-29b-3p、miR-29c-3p、miR-126-3p、miR-129-1-3p、miR-137、miR-192-5p、miR-212-3p 和 miR-320c 与 DN 的关系。
研究纳入 50 例健康对照者和 100 例 2 型糖尿病患者。糖尿病患者根据尿白蛋白排泄率（UAER）分为三组：正常白蛋白尿组（A1，n=51）、微量白蛋白尿组（A2，n=25）和大量白蛋白尿组（A3，n=24）。采用罗氏 Cobas 8000 全自动生化分析仪检测生化指标，采用实时荧光定量聚合酶链式反应（RT-qPCR）法检测血浆 miRNA 的相对表达水平。
与对照组相比，大量白蛋白尿组（A3，p=0.005，降低 6.6 倍）和糖尿病肾病组（A1+A3，p=0.005，降低 6.6 倍）miR-21-3p 的相对表达水平显著降低。与对照组相比，糖尿病肾病组 miR-192-5p 的相对表达水平也显著降低（p=0.027，降低 2.4 倍）。miR-21-3p 用于区分糖尿病肾病组和对照组的曲线下面积（AUC）值为 0.726，miR-192-5p 的 AUC 值为 0.717。
miR-21-3p 和 miR-192-5p 的表达降低与 DN 的发生发展相关，可能是早期诊断 DN 的潜在生物标志物。
